1alpha,25(OH)2D3 regulation of integrin expression is substrate dependent

Journal of Biomedical Materials Research. Part a
Perry RazZvi Schwartz

Abstract

Osteoblasts are attachment-dependent cells that interact with their surface through integrin-mediated mechanisms. Their differentiation is regulated by 1,25-dihydroxyvitamin D3 [1alpha,25(OH)(2)D(3)] and is affected by substrate chemistry and microtopography, suggesting that 1alpha,25(OH)(2)D(3) may regulate integrin expression in a surface-specific manner. To test this hypothesis, osteoblast-like human MG63 cells were grown on tissue culture plastic and on grit-blasted and acid-etched titanium disks with a complex microtopography to induce osteoblast differentiation. Expression of alpha(2), alpha(5), alpha(v), beta(1), and beta(3) integrins were quantified by real-time polymerase chain reaction (PCR) as a function of time in culture and treatment with 1alpha,25(OH)(2)D(3). Results were correlated with expression of osteocalcin, a marker of a differentiated osteoblast. Osteocalcin mRNA increased with time and 1alpha,25(OH)(2)D(3) treatment and these changes were greater in cultures on the titanium disks. Integrin expression varied with time in culture and this was also surface dependent. At each time point, beta(1) and alpha(2) mRNAs were greater on titanium than on plastic, whereas alpha(5) expression was reduced and alpha(v),...Continue Reading

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Citations

Apr 10, 2012·Journal of Materials Science. Materials in Medicine·Petros A KokkinosDespina D Deligianni
Oct 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·R Olivares-NavarreteZ Schwartz
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Mar 25, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Youtao XieChuanxian Ding
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Apr 24, 2018·Stem Cells International·Francesca PosaGiorgio Mori
Mar 7, 2007·Journal of Biomedical Materials Research. Part a·Z SchwartzB D Boyan
Feb 2, 2019·Journal of Biomedical Materials Research. Part a·Helena B LopesMarcio M Beloti
Apr 16, 2014·Journal of Biomedical Materials Research. Part a·Min LaiBarbara D Boyan
Aug 26, 2009·Journal of Biomedical Materials Research. Part a·Mimi FangZvi Schwartz
Sep 28, 2006·The Journal of the American Academy of Orthopaedic Surgeons·Barbara D Boyan, Zvi Schwartz

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