1H, 13C, and 15N backbone chemical shift assignments of m7GTP cap-bound Leishmania initiation factor 4E-1.

Biomolecular NMR Assignments
Anissa Belfetmi, Mélissa Léger-Abraham

Abstract

Most of the translational control of gene expression in higher eukaryotes occurs during the initiation step of protein synthesis. While this process is well characterized in mammalian cells, it is less defined in parasites, including the ones that cause human Leishmaniasis. The Leishmania cap-binding isoform 1 (LeishIF4E-1) is the only isoform that binds the specific trypanosomatids-specific hypermethylated 5' cap, called cap-4, in the human stage of the parasite life cycle. We report here the extensive NMR resonance assignment of LeishIF4E-1 bound to a cap analog, m7GTP. The chemical shift data constitute a prerequisite to understanding specific translation initiation mechanisms used in Leishmania parasites and to developing antiparasitic drugs targeting their translation initiation factors.

References

Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Sep 25, 2004·RNA·Yael YoffeMichal Shapira
Oct 13, 2006·The EMBO Journal·Laurent VolponKatherine L B Borden
Jul 19, 2011·Nucleic Acids Research·Alexandra ZinovievMichal Shapira
Feb 24, 2015·Molecular Cell·Daniel PeterElisa Izaurralde
Jul 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Naotaka SekiyamaMélissa Léger-Abraham
Mar 22, 2018·Nucleic Acids Research·Shimi MeleppattuMélissa Léger-Abraham
Apr 7, 2018·Wiley Interdisciplinary Reviews. RNA·Nikolay E Shirokikh, Thomas Preiss
May 9, 2019·Nucleic Acids Research·Lidia Watanabe ReolonBeatriz Gomes Guimarães
Oct 19, 2019·Chembiochem : a European Journal of Chemical Biology·Josef LeiterRonald Micura

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Citations

May 18, 2021·Computational and Structural Biotechnology Journal·Lilian Hernández-AlvarezFátima Pereira de Souza

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Methods Mentioned

BETA
NMR

Software Mentioned

CARA
hmsIST
SPARKY

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