PMID: 8589606Sep 1, 1995Paper

1H, 13C and 15N NMR assignments and solution secondary structure of rat Apo-S100 beta

Journal of Biomolecular NMR
J C AmburgeyDavid J Weber

Abstract

The 1H, 13C and 15N NMR assignments of the backbone and side-chain resonances of rat S100 beta were made at pH 6.5 and 37 degrees C using heteronuclear multidimensional NMR spectroscopy. Analysis of the NOE correlations, together with amide exchange rate and 1H alpha, 13C alpha and 13C beta chemical shift data, provided extensive secondary structural information. Thus, the secondary structure of S100 beta was determined to comprise four helices (Leu3-Ser18, helix I; Lys29-Leu40, helix II; Gln50-Glu62, helix III; and Phe70-Ala83, helix IV), four loops (Gly19-His25, loop I; Ser41-Glu49, loop II; Asp63-Gly66, loop III; and Cys84-Glu91, loop IV) and two beta-strands (Lys26-Lys28, beta-strand I and Glu67-Asp69, beta-strand II). The beta-strands were found to align in an antiparallel manner to form a very small beta-sheet. This secondary structure is consistent with predictions that S100 beta contains two 'helix-loop-helix' Ca(2+)-binding motifs known as EF-hands. The alignment of the beta-sheet, which brings the two EF-hand domains of S100 beta into close proximity, is similar to that of several other Ca(2+)-ion-binding proteins.

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Citations

Nov 1, 1996·Protein Science : a Publication of the Protein Society·B C PottsW J Chazin
May 1, 1997·Protein Science : a Publication of the Protein Society·S P SmithG S Shaw
Jul 1, 1997·Protein Science : a Publication of the Protein Society·A C DrohatD J Weber
Apr 16, 1998·Protein Science : a Publication of the Protein Society·P T WilderD J Weber
Dec 10, 2002·Journal of Molecular Biology·Keith G InmanDavid J Weber
Jan 15, 1998·Biochimica Et Biophysica Acta·A LandarD B Zimmer
Oct 29, 2011·Biochemistry·Brittney A ManvillaAlexander C Drohat
Jan 23, 2004·The Journal of Biological Chemistry·Martin A Wear, John A Cooper
Apr 22, 1999·Protein Science : a Publication of the Protein Society·A C DrohatD J Weber
Sep 10, 2010·Cardiovascular Psychiatry and Neurology·Danna B Zimmer, David J Weber
Jan 5, 2016·Journal of Medicinal Chemistry·Michael Christopher CavalierDavid J Weber
Sep 12, 2015·Nucleic Acids Research·Shuja S MalikAlexander C Drohat
Sep 2, 2016·Nucleic Acids Research·Christopher T CoeyAlexander C Drohat
Jul 31, 2007·The EMBO Journal·Thorsten OstendorpGünter Fritz
Jun 5, 2004·The Journal of Biological Chemistry·Jing LinDavid J Weber
May 7, 2015·Acta Crystallographica. Section D, Biological Crystallography·Jaime L JensenChristopher L Colbert
Jun 16, 2016·Acta Crystallographica. Section D, Structural Biology·Michael C CavalierDavid J Weber
Feb 13, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Srinivasaraghavan KannanChandra S Verma
Oct 1, 2014·Biochemistry·Michael C CavalierDavid J Weber
Jan 8, 2010·Journal of Molecular Biology·Thomas H CharpentierDavid J Weber

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