1H, 13C and 31P MAS NMR studies of lyophilized brain tumors

Solid State Nuclear Magnetic Resonance
R MarszałekI Wawer

Abstract

(1)H, (13)C and (31)P magic angle spinning magnetic resonance spectra (MAS NMR) of lyophilized brain tissue specimens were recorded. Among the 35 cases of brain tumors there were 24 glioblastomas, seven meningiomas and a few other types. (1)H NMR measurements were performed with a MAS speed of 33 kHz. The intense CH(3), CH(2) and CH peaks in the (1)H spectrum result from fatty acid residues of phospholipids, which are "mobile enough" besides the anhydrous environment. (13)C CPMAS spectra revealed the resonances of creatine and guanidine carbons; the high intensity signals arise from carbonyl groups and methylene carbons of lipids. In particular we found a fraction of mobile lipids, characterized by narrow resonances and long T(1rho)(H) Overlapped resonances of phospholipids head groups contributed to the peak at 4-7 ppm in the (31)P MAS NMR spectra. Our results indicate that (1)H and (13)C MAS NMR are able to characterize tumor types: differentiate glioblastomas from meningiomas and shed light on tumor biochemical characteristics. However, water soluble metabolites are not observed and macromolecules yield broad overlapped resonances. Generally, lyophilization significantly decreases discriminative potential of NMR analysis.

References

Aug 1, 2000·Trends in Biochemical Sciences·J M Hakumäki, R A Kauppinen
Apr 30, 2005·Journal of Insect Science·Tsunenori Kameda

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.