1H-13C separated local field spectroscopy of uniformly 13C labeled peptides and proteins.

Journal of Magnetic Resonance
Eugene C LinStanley J Opella

Abstract

By incorporating homonuclear decoupling on both the (1)H and (13)C channels it is feasible to obtain high-resolution two-dimensional separated local field spectra of peptides and proteins that are 100% labeled with (13)C. Dual-PISEMO (Polarization Inversion Spin Exchange Modulated Observation) can be performed as a conventional two-dimensional experiment, or with windowed detection as a one-dimensional experiment that offers flexibility as a building block for shiftless and other multidimensional triple-resonance experiments with the inclusion of (15)N irradiation. The triple-resonance MAGC probe used to perform these experiments at 500 MHz is described.

References

May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J S Waugh
May 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ana Carolina ZeriStanley J Opella
May 12, 2005·The Journal of Peptide Research : Official Journal of the American Peptide Society·N SinhaS J Opella
Jul 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·M Meselson, F W Stahl
Nov 1, 2006·Journal of Magnetic Resonance·Alexander A Nevzorov, Stanley J Opella
Nov 6, 2007·Journal of Magnetic Resonance·Chin H Wu, Stanley J Opella
Feb 13, 2008·The Journal of Chemical Physics·Chin H Wu, Stanley J Opella
May 4, 2010·Journal of Magnetic Resonance·Christopher V GrantStanley J Opella

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Citations

Jan 6, 2012·Journal of the American Chemical Society·Bibhuti B DasStanley J Opella
Dec 2, 2020·Solid State Nuclear Magnetic Resonance·Joel LapinAlexander A Nevzorov
Sep 21, 2013·The Journal of Physical Chemistry. B·Bibhuti B DasStanley J Opella

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