Aug 2, 2000

1H and (13)C NMR of multilamellar dispersions of polyunsaturated (22:6) phospholipids

Biophysical Journal
S Everts, J H Davis

Abstract

The polyunsaturated fatty acid docosahexaenoic acid (DHA) makes up approximately 50% of the lipid chains in the retinal rod outer segment disk membranes and a large fraction of the lipid chains in the membranes of neuronal tissues. There is an extensive literature concerned with the dietary requirements for essential fatty acids and the importance of DHA to human health, but relatively little research has been done on the physical properties of this important molecule. Using (1)H and (13)C MAS NMR measurements of dispersions of 1-palmitoyl-2-docosahexaenoyl-phosphatidylcholine in excess phosphate buffer, we have unambiguously assigned most of the resonances in both the (1)H and (13)C NMR spectra. We were able to use cross-polarization spectroscopy to follow the transfer of polarization from specific (1)H nuclei not only to their directly bonded (13)C but also to those (13)C that are in close proximity, even though they are not directly bonded. Cross-peaks in two-dimensional cross-polarization spectra revealed a close association between the choline headgroup and at least part of the DHA chain but not with the palmitate chain. Finally, we examined the dynamics of the different parts of this lipid molecule, using rotating frame s...Continue Reading

Mentioned in this Paper

Muscle Rigidity
Fluctuation
Choline
In Vivo NMR Spectroscopy
Torsion (Malposition)
RHO gene
Docosahexaenoic acid
Phosphate buffers
Tissue Membrane
Physiologic Pulse

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