¹H and hyperpolarized ³He magnetic resonance imaging clearly detect the preventative effect of a glucocorticoid on endotoxin-induced pulmonary inflammation in vivo

Innate Immunity
Lars E OlssonPaul D Hockings

Abstract

Proton (¹H ) magnetic resonance imaging (MRI) can be utilized to quantify pulmonary edema in endotoxin-induced pulmonary inflammation and hyperpolarized (HP) ³He MRI can assess pulmonary ventilation. Neither of the methods has been applied to assess the impact of a drug on endotoxin-induced pulmonary inflammation in vivo. The aim of the current study was to evaluate the capability of ¹H and HP ³He MRI to assess the effects of a glucocorticoid on endotoxin-induced pulmonary inflammation in vivo. Mice were exposed to an aerosol of either saline or endotoxin (5 mg/ml) for 10 min. Half of the endotoxin-exposed mice were pretreated with a glucocorticoid (budesonide 3 mg/kg; 2 times/day) and the other half with vehicle p.o. The first budesonide treatment was administered 1 h prior to the aerosol inhalation. Forty-eight hours after the aerosol exposure, the mice were anaesthetized for subsequent imaging. Hyperpolarized ³He was administered and axial MR images of the lungs obtained. Matching ¹H MR images were then acquired. The mice were sacrificed and broncho-alveolar lavage (BAL) samples were harvested to determine total and cell differential counts. The lesion volume on both ¹H and ³He MRI, were markedly increased by endotoxin expos...Continue Reading

References

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Mar 8, 2008·Pulmonary Pharmacology & Therapeutics·Joanne L Wright, Andrew Churg
Apr 3, 2008·Journal of Magnetic Resonance Imaging : JMRI·Yannick CrémillieuxJean-Marc Pequignot
Mar 24, 2009·Journal of Magnetic Resonance Imaging : JMRI·Lars E OlssonPaul D Hockings

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