1H HR-MAS NMR of carotenoids in aqueous samples and raw vegetables

Magnetic Resonance in Chemistry : MRC
M L Miglietta, R Lamanna

Abstract

Carotenoids are linear C40 tetraterpenoid hydrocarbons and represent a wide category of natural pigments. They are components of the pigment system of chloroplasts and are involved in the primary light absorption and the photon canalization of photosynthesis. Moreover, they also behave as quenchers of singlet oxygen, protecting cells and organisms against lipid peroxidation. Carotenoids have a strong lipophilic character and are usually analyzed in organic solvents. However, because of their biological activity, the characterization of these compounds in an aqueous environment or in the natural matrix is very important. One of the most important dietary carotenoids is beta-carotene, which has been extensively studied both in vivo and in model systems, but because of the low concentration and strong interaction with the biological matrix, beta-carotene has never been observed by NMR in solid aqueous samples.In the present work, a model system has been developed for the detection and identification of beta-carotene in solid aqueous samples by 1H HR-MAS NMR. The efficiency of the model has led to the identification of beta-carotene in a raw vegetable matrix.

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Citations

Aug 23, 2012·Environmental Science & Technology·Andre J SimpsonRonald Soong
Jan 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Navratna VajpaiStephan Grzesiek
Jul 14, 2010·Plant Physiology and Biochemistry : PPB·Marina LauraMonica Mattana
Oct 18, 2011·Magnetic Resonance in Chemistry : MRC·Masanori KodaMasaru Tanokura
Feb 9, 2012·Magnetic Resonance in Chemistry : MRC·Massimiliano ValentiniPaolo Sequi
Mar 19, 2014·Magnetic Resonance in Chemistry : MRC·Roksana WałęsaMałgorzata A Broda
Apr 3, 2020·Magnetic Resonance in Chemistry : MRC·Yalda Liaghati MobarhanAndre J Simpson
Aug 15, 2018·Analytical and Bioanalytical Chemistry·Paris NingAndré J Simpson
Mar 15, 2011·Progress in Nuclear Magnetic Resonance Spectroscopy·André J SimpsonMyrna J Simpson

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