1H-HRMAS NMR study of smoked Atlantic salmon (Salmo salar)

Magnetic Resonance in Chemistry : MRC
David CastejónAntonio Herrera

Abstract

High-resolution magic angle spinning (HRMAS) NMR spectroscopic data of smoked Atlantic salmon (Salmo salar) were fully assigned by combination of one- and two-dimensional-HRMAS experiments. Complete representative spectra, obtained after few minutes of analysis time, revealed a large number of minor and major compounds in the sample. The methodology is limited by the low sensitivity of NMR, and therefore HRMAS only enables the determination of the most relevant components. These were fatty acids (FAs), carbohydrates, nucleoside derivatives, osmolytes, amino acids, dipeptides and organic acids. For the first time, spectra were resolved sufficiently to allow semiquantitative determination in intact muscle of the highly polyunsaturated FA 22:6 omega-3. Additionally, the feasibility of (1)H-HRMAS NMR metabolite profiling was tested to identify some bioactive compounds during storage. This profiling was carried out by the non-destructive and direct analysis (i.e. without requiring sample preparation and multiple step procedures) of intact salmon muscle. The proposed procedure can be applied to a large number of samples with high throughput due to the short time of analysis and quick evaluation of the data.

References

Jul 13, 2006·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·John S AllenHanna Damasio
Sep 15, 2009·Trends in Plant Science·Andrea T Kramer, Kayri Havens

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Citations

Nov 29, 2015·Analytica Chimica Acta·David CastejónMaría Isabel Cambero
Feb 19, 2014·International Journal of Cancer. Journal International Du Cancer·Elif B UnterbergerMichael Schwarz
Jun 9, 2018·Proteomics·Marta GallegoFidel Toldrá
Oct 18, 2011·Magnetic Resonance in Chemistry : MRC·Masanori KodaMasaru Tanokura
Feb 9, 2012·Magnetic Resonance in Chemistry : MRC·Massimiliano ValentiniPaolo Sequi
Jun 8, 2012·Magnetic Resonance in Chemistry : MRC·Ulf EriksonMarit Aursand
Apr 30, 2013·Magnetic Resonance in Chemistry : MRC·Palmira VillaAntonio Herrera
Mar 15, 2019·Metabolomics : Official Journal of the Metabolomic Society·Henrik Max Jensen, Hanne Christine Bertram

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