1H magnetic resonance spectroscopy metabolites as biomarkers for cell cycle arrest and cell death in rat glioma cells.

The International Journal of Biochemistry & Cell Biology
Ladan MirbahaiAndrew C Peet

Abstract

Improved non-invasive imaging biomarkers of treatment response contribute to optimising cancer management and metabolites detected by proton magnetic resonance spectroscopy ((1)H MRS) show promise in this area. Understanding (1)H MRS changes occurring in cells during cell stress and cell death in vitro should aid the selection of pertinent biomarkers for clinical use. BT4C glioma cells in culture were exposed to either 50 μM cis-dichlorodiammineplatinum II (cisplatin) or starvation by culture in phosphate buffered saline. High resolution magic angle spinning (1)H MRS was performed on cells using a Varian 600 MHz nanoprobe and metabolites were quantified by a time domain fitting method. Cell viability was assessed by trypan blue, H&E, 4',6-diamino-2-phenylindole (DAPI), DNA laddering and annexin V-FITC labelled flow cytometry; propidium iodide flow cytometry was used to assess the cell cycle phase. With cisplatin exposure, cells initially accumulated in the G1 stage of the cell cycle with low numbers of apoptotic and necrotic cells and this was associated with decreases in phosphocholine, succinate, alanine, taurine, glycine and glutamate and increases in lactate and glycerophosphocholine (GPC). Starvation, leading to necrotic c...Continue Reading

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Citations

Feb 19, 2013·Mechanisms of Ageing and Development·Claudia Gey, Karsten Seeger
Nov 4, 2015·Brain Pathology·Myriam M ChaumeilSabrina M Ronen
Mar 1, 2017·Journal of Proteome Research·Inês LamegoAna M Gil
Jan 1, 2014·Electrophoresis·Adriana Z BuzattoAna Valéria C Simionato
Sep 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Federica De CastroFrancesco Paolo Fanizzi
Jan 6, 2021·Amino Acids·Ernest Jan BobeffDariusz Jan Jaskólski
Jun 3, 2021·Metabolites·Karol JarochBarbara Bojko

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