Abstract
Metabolic characteristics of colon mucosa, submucosa, muscularis and tumour specimens from four control (n = 105) and nine carcinogen (azoxymethane)-treated (n = 91) Sprague-Dawley rats were investigated by ex vivo 1H MRS. Ninety-seven per cent of pure mucosa samples (n = 59) yielded spectra with narrow lipid resonances (chemical shift delta of -(CH2)n-, 1.3 ppm; linewidth at half-height v1/2, 30-50 Hz). Eighty-two per cent of control mucosa samples with histologically proven submucosa contamination (n = 11) and 46% of control cross-sections (containing mucosa, submucosa and muscularis; n = 57) yielded spectra with broad lipid resonances (delta-(CH2)n-, 1.5 ppm; v1/2, 80-100 Hz) identical to those of adipose tissue surrounding rat colon. Thirty per cent of tumour samples (n = 10) yielded spectra with narrow lipid resonances while 70% contained no significant amount of MR visible lipids. We conclude that (i) lipids giving rise to broad resonances are in the heterogeneously distributed adipocytes of submucosa, (ii) lipids giving rise to narrow resonances are within the mucosa in an unknown structural environment, and (iii) the type and distribution of lipids in human and rat colon are similar. Tumours contained significantly more...Continue Reading
References
Sep 1, 1992·NMR in Biomedicine·W Negendank
Nov 17, 1992·Biochemistry·C L LeanC E Mountford
Oct 1, 1992·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·A C KueselI C Smith
Mar 1, 1992·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J Peeling, G Sutherland
Mar 1, 1991·Human Pathology·L RoncucciW R Bruce
Nov 1, 1991·Carcinogenesis·E A McLellanR P Bird
Feb 1, 1991·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·A BouchardG M Pohost
Jan 1, 1990·Virchows Archiv. A, Pathological Anatomy and Histopathology·K SekikawaF T Bosman
Aug 1, 1990·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·D Sappey-Marinier
Sep 1, 1990·Biochimica Et Biophysica Acta·D Y Sze, O Jardetzky
Oct 30, 1987·Cancer Letters·R P Bird
May 5, 1986·Life Sciences·P K Rangachari, D McWade
Sep 1, 1984·Journal of Biochemical and Biophysical Methods·C E MountfordI C Smith
Dec 4, 1984·Biochemistry·K J CrossP E Wright
Oct 15, 1994·International Journal of Cancer. Journal International Du Cancer·W B MackinnonC E Mountford
Jun 1, 1994·NMR in Biomedicine·A C KueselI C Smith
May 1, 1994·NMR in Biomedicine·A C KueselI C Smith
Dec 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·L C StewartJ F Nédélec
Nov 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·C L LeanC E Mountford
Sep 1, 1993·Radiology·E J DelikatnyC E Mountford
Mar 1, 1993·NMR in Biomedicine·A MorenoC Arús
Jan 1, 1993·CA: a Cancer Journal for Clinicians·C C BoringT Tong
Feb 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·F SchickO Lutz
Apr 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·R CalliesK M Brindle
Citations
Feb 1, 1996·NMR in Biomedicine·A Moreno, C Arús
Jun 1, 1996·NMR in Biomedicine·D EndeC E Mountford
Dec 24, 1997·Journal of Magnetic Resonance Imaging : JMRI·D E SaundersM M Brown
Aug 20, 2010·Lipids·Sonal VarmaTedros Bezabeh
Nov 28, 2007·Journal of Inflammation·Sonal VarmaTedros Bezabeh
May 19, 2010·Biomarkers in Medicine·G A Nagana GowdaTedros Bezabeh
Apr 17, 2012·Magnetic Resonance Imaging·Min Ju KimSeong Hyeon Yun
May 4, 2011·NMR in Biomedicine·E James DelikatnyHarish Poptani
Mar 10, 2001·The American Journal of Gastroenterology·T BezabehC N Bernstein
Jun 3, 1998·NMR in Biomedicine·L McIntoshJ E Anderson
Feb 3, 2007·Journal of Proteome Research·Julian L Griffin, Risto A Kauppinen