PMID: 8612608Mar 1, 1996Paper

1H-NMR-derived secondary structure and the overall fold of the potent anti-mammal and anti-insect toxin III from the scorpion Leiurus quinquestriatus quinquestriatus

European Journal of Biochemistry
C LandonM Ptak

Abstract

We describe the secondary structure and the overall fold of toxin III from the venom of the scorpion Leiurus quinquestriatus quinquestriatus determined using two-dimensional-1H-NMR spectroscopy. This protein, which contains 64 amino acids and 4 disulfide bridges, belongs to the long-chain toxin category and is highly toxic to both mammals and insects. The overall fold was determined on the basis of 1208 inter-proton-distance restraints derived from NOE measurements and 90 psi, phi dihedral-angle restraints derived from NOE connectivities and 3JNH-alphaH coupling constants using the HABAS program. This fold, which mainly consists of an alpha-helix packed against a small antiparallel three-stranded beta-sheet, and of several turns and loops, is similar to that of other long-chain scorpion toxins. Aromatic and non-polar residues form several patches on the surface of the protein which alternate with patches of charged and polar residues. Such a topology should be important in the interactions of toxin III with sodium channels in membranes. Two weakly constrained loops introduce some flexibility to the structure which could be related to the activity of this toxin. The central core of toxin III is compared with the cysteine-stabili...Continue Reading

References

Sep 5, 1992·Journal of Molecular Biology·B ZhaoC E Bugg
Jul 1, 1991·Journal of Biomolecular NMR·C EcclesK Wüthrich
Jan 1, 1990·Toxicon : Official Journal of the International Society on Toxinology·R KharratH Rochat
Aug 29, 1986·Biochemical and Biophysical Research Communications·M E De LimaH Rochat
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J C Fontecilla-CampsH Rochat
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A T BrüngerM Karplus
Sep 16, 1974·European Journal of Biochemistry·C KopeyanH Rochat
Jan 1, 1982·Toxicon : Official Journal of the International Society on Toxinology·F CouraudH Rochat
Jan 1, 1983·Toxicon : Official Journal of the International Society on Toxinology·J Gregoire, H Rochat
Oct 1, 1982·International Journal of Peptide and Protein Research·H DarbonH Rochat
Mar 1, 1994·Proteins·M D Pagel, D E Wemmer
Apr 22, 1994·Journal of Molecular Biology·D HoussetJ C Fontecilla-Camps
Jan 15, 1994·European Journal of Biochemistry·W LeeN R Krishna
Jan 1, 1993·Progress in Biophysics and Molecular Biology·A V Efimov
Jul 1, 1995·Journal of Biomolecular NMR·C BartelsK Wüthrich

❮ Previous
Next ❯

Citations

Sep 23, 1997·Protein Science : a Publication of the Protein Society·C LandonM Ptak
Dec 7, 2002·Toxicon : Official Journal of the International Society on Toxinology·Tamer I Zaki, James E Maruniak
Oct 29, 1998·Toxicon : Official Journal of the International Society on Toxinology·M GurevitzN Chejanovsky
Oct 18, 2002·The Journal of Peptide Research : Official Journal of the American Peptide Society·Y-M SunD-C Wang
Feb 12, 2005·Insect Biochemistry and Molecular Biology·Tal ArnonEliahu Zlotkin
Jun 8, 2006·Journal of Biochemistry and Molecular Biology·Maryam NikkhahMohamad N Sarbolouki
Nov 14, 1997·The Journal of Biological Chemistry·D DestoumieuxE Bachère
May 3, 2013·The Journal of Biological Chemistry·Anton O ChugunovRoman G Efremov
Oct 9, 2013·Acta Crystallographica. Section D, Biological Crystallography·Monika A CoronadoChristian Betzel
Sep 28, 2016·Protein Engineering, Design & Selection : PEDS·Shangfei ZhangShunyi Zhu
Jun 6, 2000·American Journal of Physiology. Cell Physiology·J I Kourie, A A Shorthouse
Jan 20, 2005·The Biochemical Journal·Najet Srairi-AbidMohamed El Ayeb

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.