2-Acetyl-1-pyrroline augmentation in scented indica rice (Oryza sativa L.) varieties through Δ(1)-pyrroline-5-carboxylate synthetase (P5CS) gene transformation

Applied Biochemistry and Biotechnology
Kayghobad KaikavoosiAltafhusain B Nadaf

Abstract

2-Acetyl-1-pyrroline (2AP) has been identified as a principal aroma compound in scented rice varieties. Δ(1)-Pyrroline-5-carboxylate synthetase (P5CS) gene is reported to regulate the proline synthesis in plants and acts as the precursor of 2AP. Two scented indica rice varieties, namely Ambemohar 157 and Indrayani, were subjected to Agrobacterium tumefaciens-mediated genetic transformation containing P5CS gene. Overexpression of P5CS led to a significant increase in proline, P5CS enzyme activity and 2AP levels in transgenic calli, vegetative plant parts, and seeds over control in both the varieties. 2AP level increased more than twofold in transgenic seeds in both varieties. This is the first report of enhancement in 2AP content through overexpression of using P5CS gene, indicating the role of proline as a precursor amino acid in the biosynthesis of 2AP in scented rice.

References

Jan 1, 1976·Journal of Agricultural and Food Chemistry·R W Bullard, G Holguin
Feb 15, 2001·Journal of Agricultural and Food Chemistry·C C GrimmR Bryant
Mar 23, 2001·Journal of Agricultural and Food Chemistry·S MahatheeranontK Dumri
Mar 21, 2002·Journal of Agricultural and Food Chemistry·Tadashi YoshihashiHideo Inatomi
Nov 30, 2006·Plant Biotechnology Journal·Louis M T BradburyDaniel L E Waters
Jan 4, 2008·BMC Plant Biology·Karsten OelkersTancred Frickey
May 21, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·F BourgisA Ghesquière
Jun 1, 1996·Plant Cell Reports·H RashidK Hinata

❮ Previous
Next ❯

Citations

Jul 5, 2016·Journal of the Science of Food and Agriculture·Kantilal WakteRobert Henry
Dec 15, 2015·Journal of Proteomics·Tarinee TimabudSetsuko Komatsu
May 4, 2017·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Usmangani AttarAltafhusain Nadaf
Jan 22, 2021·Journal of Biotechnology·Harshal V DhondgeAltafhusain B Nadaf
Jul 3, 2021·International Journal of Molecular Sciences·Vacha BhattAltafhusain Nadaf

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.