β2-Adrenergic Receptors Increase Cardiac Fibroblast Proliferation Through the Gαs/ERK1/2-Dependent Secretion of Interleukin-6.

International Journal of Molecular Sciences
Miles A TannerLaurel A Grisanti

Abstract

Fibroblasts are an important resident cell population in the heart involved in maintaining homeostasis and structure during normal conditions. They are also crucial in disease states for sensing signals and initiating the appropriate repair responses to maintain the structural integrity of the heart. This sentinel role of cardiac fibroblasts occurs, in part, through their ability to secrete cytokines. β-adrenergic receptors (βAR) are also critical regulators of cardiac function in the normal and diseased state and a major therapeutic target clinically. βAR are known to influence cytokine secretion in various cell types and they have been shown to be involved in cytokine production in the heart, but their role in regulating cytokine production in cardiac fibroblasts is not well understood. Thus, we hypothesized that βAR activation on cardiac fibroblasts modulates cytokine production to influence fibroblast function. Using primary fibroblast cultures from neonatal rats and adult mice, increased interleukin (IL)-6 expression and secretion occurred following β2AR activation. The use of pharmacological inhibitors and genetic manipulations showed that IL-6 elevations occurred through the Gαs-mediated activation of ERK1/2 and resulted...Continue Reading

References

Mar 11, 1999·Journal of Receptor and Signal Transduction Research·C A IzeboudR F Witkamp
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·S EngelhardtM J Lohse
Nov 26, 1999·Circulation Research·R P XiaoE G Lakatta
Jan 7, 2000·Biochemical and Biophysical Research Communications·S DremierC Maenhaut
Oct 31, 2000·Cardiovascular Research·M LeichtH Zimmer
Apr 20, 2001·Heart Failure Reviews·K C Wollert, H Drexler
May 8, 2001·Molecular and Cellular Biology·J M Schmitt, P J Stork
Jun 19, 2001·American Journal of Physiology. Heart and Circulatory Physiology·A BürgerH G Zimmer
Jan 24, 2002·Molecular Cell·John M Schmitt, Philip J S Stork
Mar 19, 2002·The Journal of Biological Chemistry·Nicolas Laroche-JoubertAlain Doucet
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Fernando Ribeiro-NetoDaniel L Altschuler
Jun 21, 2002·Trends in Cell Biology·Philip J S Stork, John M Schmitt
Oct 3, 2002·Current Opinion in Critical Care·Marc LeoneClaude Martin
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·George S BaillieMiles D Houslay
Sep 24, 2004·Cellular Signalling·Negin P MartinRobert J Lefkowitz
Jul 13, 2005·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Christine E Pullar, R Rivkah Isseroff
Nov 11, 2005·The Journal of Biological Chemistry·Sudha K ShenoyRobert J Lefkowitz
Jan 7, 2006·The Journal of Investigative Dermatology·Randle M GallucciJames J Tomasek
Aug 11, 2006·Arthritis Research & Therapy·Tadamitsu Kishimoto
Nov 30, 2006·Annals of the New York Academy of Sciences·Indroneal BanerjeeTroy A Baudino
May 5, 2007·BMJ : British Medical Journal·H T Ong
Feb 17, 2009·Developmental Cell·Masaki IedaDeepak Srivastava
May 23, 2009·Pharmacology & Therapeutics·Karen E Porter, Neil A Turner
Oct 6, 2009·Journal of Biomechanics·Boris Hinz
Dec 5, 2009·Circulation Research·Colby A SoudersTroy A Baudino
Feb 2, 2010·Molecular Immunology·Laurel A GrisantiJames E Porter
Jul 20, 2010·Biochimica Et Biophysica Acta·Pablo Aránguiz-UrrozGuillermo Díaz-Araya
Jul 23, 2011·Genes & Cancer·Inbal Wortzel, Rony Seger

❮ Previous
Next ❯

Citations

Apr 3, 2021·Molecular Biology Reports·Tapan BehlSwati Chadha
Aug 28, 2021·International Journal of Molecular Sciences·Paola MatarreseGiuseppe Marano
Aug 21, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Miles A TannerLaurel A Grisanti
Sep 17, 2021·Frontiers in Cardiovascular Medicine·Miles A Tanner, Laurel A Grisanti

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
biopsy
nucleotide exchange
PCR
biopsies
Assay
scraping

Software Mentioned

ImageJ
GraphPad
Prism

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.