PMID: 11921195Mar 29, 2002Paper

2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)-induced mutagenesis in cultured Big Blue rat mammary epithelial and fibroblast cells

Environmental and Molecular Mutagenesis
H M McDiarmidP David Josephy

Abstract

Epithelial cells are the primary site of carcinogenesis in most tissues, including the mammary gland. As an alternative to the study of mutation induction in whole tissues in vivo, we have established Big Blue transgenic rat cell lines from the mammary epithelium (BBR/ME) and the mammary stroma (BBR/MFib), to permit a comparison of their mutagenic responses to carcinogens. We previously demonstrated their responsiveness to the alkylating agent N-ethyl-N-nitrosourea (ENU) (McDiarmid H et al. [2001]: Mutat Res 497:39-47). Here, we examined the responses of cultured epithelial and stromal cells to the protein pyrolysis product and mammary carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Rat hepatic S9 was used as a source of bioactivation enzymes. Mutant induction (cII locus) and clonogenic survival were measured as a function of PhIP concentration. PhIP mutagenicity was observed in the fibroblast cells, but the greater toxicity of PhIP to the epithelial cells prevented a definitive evaluation of mutagenicity. Since PhIP may be detoxified by conjugation with glutathione, we measured glutathione levels and glutathione-S-transferase expression and activities in both cell lines. The epithelial cells had higher gluta...Continue Reading

References

Dec 1, 1989·Japanese Journal of Cancer Research : Gann·H EsumiT Sugimura
Jan 1, 1981·Methods in Enzymology·W H Habig, W B Jakoby
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·K BerhaneB Mannervik
Jan 27, 1998·Archives of Toxicology. Supplement. = Archiv Für Toxikologie. Supplement·P SchmezerH Bartsch
Jun 4, 1999·Mutation Research·B SunJ A Heddle
Apr 5, 2001·American Journal of Physiology. Endocrinology and Metabolism·Y H Kwon, M H Stipanuk
Apr 27, 2001·Toxicology Letters·R C SillsM L Cunningham
Nov 20, 2001·Chemical Research in Toxicology·L S DeBruinP D Josephy

❮ Previous
Next ❯

Citations

Jul 3, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Michelle Scrivens, Nirmala Bhogal
Dec 3, 2009·Environmental and Molecular Mutagenesis·Guosheng ChenPaul A White
Aug 6, 2005·Mutation Research·Iain B LambertGeorge R Douglas
Oct 14, 2003·Environmental and Molecular Mutagenesis·Paul A WhiteDavid Blakey
Jan 18, 2018·International Journal of Molecular Sciences·Ahmad BesaratiniaStella Tommasi
Apr 26, 2005·International Journal of Cancer. Journal International Du Cancer·Erzsébet Papp-SzabóBrenda L Coomber
Mar 6, 2010·Molecular and Cellular Biochemistry·Sutapa MukhopadhyaySalil K Das

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.