β2-AR-induced Her2 transactivation mediated by Erbin confers protection from apoptosis in cardiomyocytes

International Journal of Cardiology
M ShiN Guo

Abstract

Her2 and β2-adrenergic receptor (β2-AR) can form a heterocomplex in cardiomyocytes and agonists can induce Her2 transactivation, which is important for cardiovascular homeostasis. The scaffolding molecules that mediate β2-AR/Her2 interaction are currently unknown. Erbin, a PDZ domain-containing protein is a binding partner of Her2. The C-terminus of β2-AR harbors a PDZ domain-binding motif. Hypothesis of this study is that Erbin may organize the assembly of β2-AR/Her2 complex. The interaction among β2-AR, Her2 and Erbin was investigated in COS-7, HEK-293 and H9c2 cells and rat brain and heart tissues by coimmunoprecipitation. The β2-AR binding region of Erbin was identified by utilizing the Erbin deletion mutants. The functional significance of Erbin in cardiomyocytes was determined by Erbin silencing, contraction frequency measurement and cellular apoptosis assays. Erbin was able to form a complex with both exogenous and endogenous β2-AR and Her2 in the presence of isoproterenol (ISO). Deletion of the Erbin LRR domain did not affect its binding to β2-AR and Her2, whereas lacking of the PDZ domain lost the ability of Erbin. Silencing of Erbin greatly abrogated ISO-induced activation of ERK. The treatment of H9c2 cells transfect...Continue Reading

References

May 2, 2002·Nature Medicine·Steven A CroneKuo-Fee Lee
Jun 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Cemil OzcelikAlistair N Garratt
Oct 16, 2002·The Journal of Biological Chemistry·Yang Z HuangLin Mei
Nov 15, 2003·Circulation Research·Martin J LohseThomas Eschenhagen
Oct 6, 2005·The Journal of Biological Chemistry·Christine McDonaldGabriel Nuñez
Nov 23, 2005·The Journal of Biological Chemistry·Penggao DaiLin Mei
Oct 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Alejandra NegroKuo-Fen Lee
Mar 23, 2007·Biochimica Et Biophysica Acta·Natasha C SalazarHoward A Rockman
Jun 27, 2007·Molecular and Cellular Biology·Fangyan DaiXin-Hua Feng
Sep 6, 2007·The Journal of Clinical Investigation·Stefan Engelhardt
Sep 6, 2007·The Journal of Clinical Investigation·Takahisa NomaHoward A Rockman
Dec 17, 2009·Endocrinology·Maria FlorianJolanta Gutkowska
Jan 18, 2011·Current Protein & Peptide Science·Liu DanNing Guo
Jul 21, 2011·Current Topics in Membranes·Weizhong ZhuRui-Ping Xiao
Nov 26, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Qiaodan ZhouGang Xu

❮ Previous
Next ❯

Citations

Mar 26, 2013·The Journal of Clinical Investigation·Robert M HarmonKathleen J Green
Apr 9, 2014·Proceedings of the National Academy of Sciences of the United States of America·Inbal RachminEhud Razin
May 6, 2019·International Journal of Molecular Sciences·Simona GalloTiziana Crepaldi
Feb 3, 2021·Molecular and Cellular Biology·Jason J Kwon, William C Hahn
May 1, 2021·Biology·Christopher J GilbertFederica Accornero
Jul 26, 2021·Cellular Signalling·Srikanth PalanisamyKathleen Gabrielson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
Chun-jiao SongYan-xin Liu
Biochemistry
Tatyana MamonovaPeter A Friedman
Matrix Biology : Journal of the International Society for Matrix Biology
Haquima El MourabitMonique Aumailley
© 2021 Meta ULC. All rights reserved