2-Chloroadenosine but not adenosine induces apoptosis in rheumatoid fibroblasts independently of cell surface adenosine receptor signalling

British Journal of Pharmacology
Masahiro KoshibaShunichi Kumagai

Abstract

1. The apoptotic effect of adenosine and its analogues was studied in fibroblast-like synoviocytes derived from rheumatoid arthritis patients (RA-FLSs). Evoked cell death was quantitatively examined by assessing DNA fragmentation using an enzyme-liked immunosorbent assay and by measuring phosphatidylserine exposure through flow cytometric analysis of annexin V binding. 2. Exposing cells for 24 h to 2-chloroadenosine (2-CADO), a nonspecific, adenosine deaminase (ADA)-resistant, adenosine receptor (AdoR) agonist, induced DNA fragmentation, and thus apoptosis, in RA-FLSs at concentrations > or =50 microM. By contrast, incubation with adenosine for up to 72 h did not evoke DNA fragmentation, even in the presence of ADA inhibitor coformycin and nucleoside transporter inhibitor nitrobenzylmercaptopurin (NBMPR). Transcription of all four AdoR isoforms was detected in RA-FLSs; nevertheless selective AdoR agonists similarly failed to induce DNA fragmentation. 3. DNA fragmentation evoked by 2-CADO was inhibited by NBMPR and by 5'-iodotubercidin, an adenosine kinase inhibitor, but not by xanthine amine congener, an A(1) and A(2) receptor antagonist, or by selective AdoR antagonists. 4. The nonspecific caspase inhibitor benzyloxycarbonyl-V...Continue Reading

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Citations

Aug 15, 2013·Purinergic Signalling·Geoffrey BurnstockIsabel R Orriss
Feb 22, 2011·American Journal of Physiology. Heart and Circulatory Physiology·John T FassettRobert J Bache
Feb 6, 2004·Biochemical Pharmacology·Nadia D'AmbrosiCinzia Volonté
Jun 10, 2005·Journal of Biochemistry and Molecular Biology·Wentian SunChee Hong Tan
Feb 5, 2008·Biochemical Pharmacology·Laurent Bastin-CoyetteFrançoise Bontemps
Mar 13, 2003·Arthritis and Rheumatism·Yuji NakamachiShunichi Kumagai
Oct 27, 2004·The Biochemical Journal·Meaghan StolkJames R Hammond
Nov 15, 2018·Frontiers in Immunology·Andrea M ChambersSandro Matosevic
Feb 11, 2020·Frontiers in Pharmacology·Carmen Corciulo, Bruce N Cronstein

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