PMID: 9171413Jun 1, 1997Paper

2-chloromuconate and ClcR-mediated activation of the clcABD operon: in vitro transcriptional and DNase I footprint analyses

Journal of Bacteriology
S M McFallA M Chakrabarty

Abstract

In Pseudomonas putida, the plasmid-borne clcABD operon encodes enzymes involved in 3-chlorocatechol degradation. Previous studies have demonstrated that these enzymes are induced when P. putida is grown in the presence of 3-chlorobenzoate, which is converted to 3-chlorocatechol, and that ClcR, a LysR-type regulator, is required for this induction. The clcABD operon is believed to have evolved from the chromosomal catBCA operon, which encodes enzymes that utilize catechol and is regulated by CatR. The inducer for the catBCA operon is an intermediate of the catechol pathway, cis,cis-muconate. In this study, we demonstrate by the use of in vitro transcription assays and lacZ transcription fusions in vivo that the analogous intermediate of the 3-chlorocatechol pathway, 2-chloromuconate, is the inducer of the clcABD operon. The DNase I footprints of ClcR with and without 2-chloromuconate were also determined. An extended region of the promoter from -79 to -25 was occupied in the absence of inducer, but the -35 region was unprotected. When 2-chloromuconate was added to the binding assays, the footprint contracted approximately 4 bp at the proximal end of the promoter, and the -35 region was contacted. It is interesting to note that C...Continue Reading

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Citations

Dec 23, 2004·Applied Microbiology and Biotechnology·Dietmar H Pieper
Sep 2, 2006·Journal of Industrial Microbiology & Biotechnology·Zhongqi HeGlenn R Johnson
Jun 23, 2006·Analytical and Bioanalytical Chemistry·Jessika FelicianoSylvia Daunert
Apr 22, 2010·Environmental Microbiology·Roberto A Bobadilla FazziniVitor A P Martins Dos Santos
Apr 16, 2019·Frontiers in Microbiology·Inna A Suvorova, Mikhail S Gelfand
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Becky M BundyEllen L Neidle
Aug 6, 2003·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Naoto OgawaA M Chakrabarty
Feb 3, 2005·Archives of Microbiology·Monika ThielMichael Schlömann
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·David Tropel, Jan Roelof van der Meer
Oct 6, 1998·Applied and Environmental Microbiology·T PotrawfkeR M Wittich
Feb 13, 2021·The FEBS Journal·Evdokia-Anastasia GiannopoulouToshiya Senda
Apr 10, 2007·Burns : Journal of the International Society for Burn Injuries·C S SobralL M Ferreira

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