2-D RP/RPLC method to separate components in Fructus schisandrae chinensis

Journal of Separation Science
Gaowa JinXinmiao Liang

Abstract

An off-line 2-D RP/RPLC method was developed to separate components in extracts of Fructus schisandrae chinensis, which is a traditional Chinese medicine. In this method, Click beta-CD stationary phase was proposed for constructing 2-D RP/RPLC system combined with C18 column. Beta-CD column showed great potential to separate compounds in traditional Chinese medicines under RP mode. Collected fractions from Click beta-CD column used in first dimension were resolved into many additional components, indicating that the 2-D LC system based on Click beta-CD and C18 phases is orthogonal. The mobile phase was same on both columns. This 2-D LC method facilitates us not only to separate components that cannot be resolved by unidimensional chromatography, but also to achieve much more efficient detection of minor sample components.

References

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Feb 29, 2008·Journal of Separation Science·Yanming LiuXinmiao Liang
Apr 23, 2008·Journal of Separation Science·Hui ZhangXinmiao Liang

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Citations

Jun 30, 2012·The American Journal of Chinese Medicine·Shaohua ZhuZhiying Huang
Aug 18, 2017·Journal of Separation Science·Fangzhou YinWu Yin
Oct 31, 2019·Journal of Separation Science·Weijia ZhouXinmiao Liang
Nov 18, 2011·Analytical and Bioanalytical Chemistry·Xiuli ZhangXinmiao Liang

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