2-DE proteomics analysis of drought treated seedlings of Quercus ilex supports a root active strategy for metabolic adaptation in response to water shortage

Frontiers in Plant Science
Lyudmila P Simova-StoilovaJesús V Jorrín-Novo

Abstract

Holm oak is a dominant tree in the western Mediterranean region. Despite being well adapted to dry hot climate, drought is the main cause of mortality post-transplanting in reforestation programs. An active response to drought is critical for tree establishment and survival. Applying a gel-based proteomic approach, dynamic changes in root proteins of drought treated Quercus ilex subsp. Ballota [Desf.] Samp. seedlings were followed. Water stress was applied on 20 day-old holm oak plantlets by water limitation for a period of 10 and 20 days, each followed by 10 days of recovery. Stress was monitored by changes in water status, plant growth, and electrolyte leakage. Contrary to leaves, holm oak roots responded readily to water shortage at physiological level by growth inhibition, changes in water status and membrane stability. Root proteins were extracted using trichloroacetate/acetone/phenol protocol and separated by two-dimensional electrophoresis. Coomassie colloidal stained gel images were analyzed and spot intensity data subjected to multivariate statistical analysis. Selected consistent spots in three biological replicas, presenting significant changes under stress, were subjected to MALDI-TOF mass spectrometry (peptide mass...Continue Reading

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Citations

Aug 10, 2019·G3 : Genes - Genomes - Genetics·Silvia MadritschEva M Sehr
Jul 28, 2018·Frontiers in Plant Science·Cristina López-HidalgoJesus V Jorrín-Novo
Feb 10, 2019·International Journal of Molecular Sciences·María-Dolores ReyJesús V Jorrín-Novo
Feb 24, 2019·International Journal of Molecular Sciences·Yuying WangYihong Ge
Mar 27, 2021·Expert Review of Proteomics·J V Jorrin Novo
May 18, 2021·Journal of Proteomics·Víctor Manuel Guerrero-SánchezMaría-Dolores Rey

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Methods Mentioned

BETA
protein folding

Software Mentioned

MASCOT
PRIDE Converter
WolfPSORT
MeV4
MultiLoc
PDQuest
NIA
GPS ExplorerTM

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