2-haloacrylate hydratase, a new class of flavoenzyme that catalyzes the addition of water to the substrate for dehalogenation.

Applied and Environmental Microbiology
Amr M MowafyN Esaki

Abstract

Enzymes catalyzing the conversion of organohalogen compounds are useful in the chemical industry and environmental technology. Here we report the occurrence of a new reduced flavin adenine dinucleotide (FAD) (FADH(2))-dependent enzyme that catalyzes the removal of a halogen atom from an unsaturated aliphatic organohalogen compound by the addition of a water molecule to the substrate. A soil bacterium, Pseudomonas sp. strain YL, inducibly produced a protein named Caa67(YL) when the cells were grown on 2-chloroacrylate (2-CAA). The caa67(YL) gene encoded a protein of 547 amino acid residues (M(r) of 59,301), which shared weak but significant sequence similarity with various flavoenzymes and contained a nucleotide-binding motif. We found that 2-CAA is converted into pyruvate when the reaction was carried out with purified Caa67(YL) in the presence of FAD and a reducing agent [NAD(P)H or sodium dithionite] under anaerobic conditions. The reducing agent was not stoichiometrically consumed during this reaction, suggesting that FADH(2) is conserved by regeneration in the catalytic cycle. When the reaction was carried out in the presence of H(2)(18)O, [(18)O]pyruvate was produced. These results indicate that Caa67(YL) catalyzes the hyd...Continue Reading

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Citations

Aug 10, 2012·Environmental Science and Pollution Research International·Ghufrana Samin, Dick B Janssen
Jul 4, 2012·Applied and Environmental Microbiology·Muhammad Irfan ArifDick B Janssen
Feb 28, 2013·International Journal of Molecular Sciences·Ismaila Yada SudiFahrul Huyop
Feb 11, 2011·Bioscience, Biotechnology, and Biochemistry·Tatsuo Kurihara
Jan 21, 2017·Chemical Reviews·Vinayak AgarwalBradley S Moore
May 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thiau-Fu AngThean Chor Leow
May 19, 2018·Chembiochem : a European Journal of Chemical Biology·Yumin DaiPablo Sobrado
Nov 28, 2020·Archives of Biochemistry and Biophysics·Pablo Sobrado
Nov 9, 2017·Chembiochem : a European Journal of Chemical Biology·Yumin DaiPablo Sobrado

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