PMID: 9187261Jun 1, 1997Paper

2-Methoxyestradiol, an endogenous estrogen metabolite, induces apoptosis in endothelial cells and inhibits angiogenesis: possible role for stress-activated protein kinase signaling pathway and Fas expression

Molecular Pharmacology
T L YueG Z Feuerstein

Abstract

2-Methoxyestradiol (2-ME) is an endogenous metabolite of estradiol-17beta and the oral contraceptive agent 17-ethylestradiol. 2-ME was recently reported to inhibit endothelial cell proliferation. The current study was undertaken to explore the mechanism of 2-ME effects on endothelial cells, especially whether 2-ME induces apoptosis, a prime mechanism in tissue remodeling and angiogenesis. Cultured bovine pulmonary artery endothelial cells (BPAEC) exposed to 2-ME showed morphological (including ultrastructural) features characteristic of apoptosis: cell shrinkage, cytoplasmic and nuclear condensation, and cell blebbing. 2-ME-induced apoptosis in BPAEC was a time- and concentration-dependent process (EC50 = 0.45 +/- 0.09 microM, n = 8). Nucleosomal DNA fragmentation in BPAEC treated with 2-ME was identified by agarose gel electrophoresis (DNA ladder) as well as in situ nick end labeling. Under the same experimental conditions, estradiol-17beta and two of its other metabolites, estriol and 2-methoxyestriol (< or =10 microM), did not have an apoptotic effect on BPAEC. 2-ME activated stress-activated protein kinase (SAPK)/c-Jun amino-terminal protein kinase in BPAEC in a concentration-dependent manner. The activity of SAPK was incre...Continue Reading

References

Jun 1, 1976·Journal of Steroid Biochemistry·H P Gelbke, R Knuppen
Apr 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L E Gerschenson, R J Rotello
Jan 1, 1987·Cell Motility and the Cytoskeleton·W J WheelerB R Brinkley
Nov 24, 1995·Science·Z XiaM E Greenberg
Mar 10, 1995·Science·S Nagata, P Golstein
Feb 1, 1995·Trends in Pharmacological Sciences·T P FanR Bicknell
Sep 1, 1995·The Journal of Experimental Medicine·Q TianP Anderson
Sep 29, 1995·The Journal of Biological Chemistry·J K WestwickD A Brenner
Nov 10, 1995·The Journal of Biological Chemistry·H SawaiH Umehara
Oct 1, 1994·Experimental Cell Research·V A PolunovskyP B Bitterman
Jul 1, 1994·Circulation Research·T L YueG Z Feuerstein
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·R J D'AmatoE Hamel
May 12, 1994·Nature·J M KyriakisJ R Woodgett
Jan 1, 1994·The Journal of Cell Biology·J C Reed
May 1, 1996·European Journal of Immunology·D J WilsonR C Budd
Jul 15, 1996·The Journal of Clinical Investigation·S StrömbladD A Cheresh
Nov 1, 1996·Stem Cells·D J McConkey, S Orrenius
Feb 1, 1995·Trends in Cell Biology·J Bischoff

❮ Previous
Next ❯

Citations

Dec 30, 2003·International Journal of Cancer. Journal International Du Cancer·Christine LambertJuergen Frank
Sep 24, 2004·International Journal of Cancer. Journal International Du Cancer·Judit DobosAndrea Ladányi
May 10, 2005·International Journal of Cancer. Journal International Du Cancer·Bindumalini RaobaikadyAtul Purohit
Jan 20, 2006·The New England Journal of Medicine·James D Yager, Nancy E Davidson
Sep 15, 2005·Journal of Cardiovascular Pharmacology·Stevan P TofovicMona F Melhem
Apr 19, 2006·Journal of Cellular Biochemistry·A MaranR T Turner
Aug 8, 2007·International Journal of Cancer. Journal International Du Cancer·Heng-Liang LinShih-Hwa Chiou
Aug 4, 2012·Circulation·Roxane Paulin, Evangelos D Michelakis
Sep 25, 2015·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Lisa RepsoldAnnie M Joubert
Nov 7, 2019·International Journal of Molecular Sciences·Tomas KoltaiStephan J Reshkin
Feb 18, 2003·Pharmacotherapy·Nehal J LakhaniWilliam D Figg
Oct 30, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Marko B LensJulia A Newton Bishop
Aug 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jing-Chuan ZhangKeith R McCrae
Nov 6, 2002·FEBS Letters·Shizhong BuMaréne Landström
Jul 11, 2006·Journal of Gastroenterology and Hepatology·Guido SchumacherPeter Neuhaus
Apr 4, 2008·Journal of Cellular Biochemistry·Avudaiappan MaranMichael J Yaszemski
Jan 9, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·D W Losordo, J M Isner
Sep 25, 2001·Journal of Applied Physiology·R K Dubey, E K Jackson
Feb 14, 2002·Journal of Applied Physiology·Amparo C VillablancaJohn C Rutledge
Aug 23, 2002·Current Opinion in Rheumatology·Joan T Merrill
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·V StellmachN Bouck
Aug 30, 2000·Journal of the National Cancer Institute. Monographs·J D Yager
May 6, 2006·Thyroid : Official Journal of the American Thyroid Association·Pernilla RoswallNils-Erik Heldin
Jul 13, 2006·Clinical Science·Shanhong LingKrishnankutty Sudhir
Dec 6, 2003·The Journal of Pharmacology and Experimental Therapeutics·Raghvendra K DubeyEdwin K Jackson
Oct 3, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Natalia V BogatchevaAlexander D Verin
Jun 5, 2011·Journal of Diabetes Investigation·Takashi YorifujiHirotaka Watada

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.