2-Methoxyestradiol Attenuates Testosterone-Induced Benign Prostate Hyperplasia in Rats through Inhibition of HIF-1α /TGF-β /Smad2 Axis

Oxidative Medicine and Cellular Longevity
Ashraf B Abdel-NaimOsama M Ashour

Abstract

Benign prostatic hyperplasia (BPH) is a common disorder in the male population. 2-Methoxyestradiol (2ME) is an end metabolite of estrogens with pleiotropic pharmacological properties. This study aimed to explore the potential ameliorative effects of 2ME against testosterone-induced BPH in rats. 2-Methoxyestradiol (50 and 100 mg/kg, dissolved in DMSO) prevented the rise in prostatic index and weight in comparison to testosterone-alone-treated animals for 2 weeks. Histological examination indicated that 2ME ameliorated pathological changes in prostate architecture. This was confirmed by the ability of 2ME to decrease the glandular epithelial height when compared to the testosterone group. Also, 2ME improved testosterone-induced oxidative stress as it inhibited the rise in lipid peroxide content and the exhaustion of superoxide dismutase (SOD) activity. The beneficial effects of 2ME against the development of BPH were substantiated by assessing proliferation markers, preventing the rise in cyclin D1 protein expression and enhancing Bax/Bcl2 mRNA ratio. It significantly reduced prostate content of tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), nuclear factor κB (NF-κB), and transforming growth factor β (TGF-β). In additio...Continue Reading

References

May 1, 1978·Analytical Biochemistry·M Mihara, M Uchiyama
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 2, 2002·Blood·Hong ZhangOlcay A Batuman
Jan 25, 2003·Journal of Biochemistry and Molecular Biology·Irfan Rahman
Oct 31, 2006·Oncogene·A HoffmannG Ghosh
Jul 31, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ahmed M Elsharkawy, Derek A Mann
Aug 8, 2007·International Journal of Cancer. Journal International Du Cancer·Heng-Liang LinShih-Hwa Chiou
Jan 9, 2008·The Journal of Endocrinology·Stina Häggström Rudolfsson, Anders Bergh
Feb 21, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Amye J TevaarwerkGlenn Liu
May 19, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Qiang ZhangChung Lee
Aug 12, 2009·The Prostate·Grégoire RobertAlexandre de la Taille
Dec 21, 2010·Current Opinion in Urology·Robert F Donnell
Dec 24, 2010·The Journal of Pharmacology and Experimental Therapeutics·F H W ShandA G Stewart
Dec 8, 2011·BJU International·Hisham A MosliAshraf B Abdel-Naim
Jan 24, 2012·Biochemical and Biophysical Research Communications·Choa ParkYoungjoo Lee
Mar 20, 2012·The Journal of Urology·Adam S BellingerUNKNOWN Urologic Diseases in America Project
Aug 7, 2012·The Journal of Biological Chemistry·Takakazu MitaniRyoichi Yamaji
Sep 19, 2012·Biochimica Et Biophysica Acta·Hye-Jin KimYang-Sook Chun
Mar 5, 2013·Reproductive Sciences·Alejandra Perez-SepulvedaSebastián E Illanes
Nov 16, 2013·Current Opinion in Urology·João SilvaFrancisco Cruz
Apr 20, 2014·Indian Journal of Urology : IJU : Journal of the Urological Society of India·Nishant D Patel, J Kellogg Parsons
Jan 27, 2015·The Prostate·Amr E AmmarEmma S Tomlinson Guns
Sep 30, 2015·The Prostate·Paz VitalMichael Ittmann
Jan 8, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jixue YangChao Wang
Aug 1, 2016·The Urologic Clinics of North America·LaTayia AaronSimon W Hayward
Sep 10, 2016·Journal of Experimental & Clinical Cancer Research : CR·Udensi K Udensi, Paul B Tchounwou

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
electrophoresis
ELISA
transcription factor assay
MDA

Software Mentioned

Gene Runner
GraphPad Instat
Macrogen
BLAST
ImageJ

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Autophagy & Aging: Inhibitors

The feed focuses on the role of nuclear export inhibitors and their effect on autophagy and the aging process.