2-o-phosphorylation of xylose and 6-o-sulfation of galactose in the protein linkage region of glycosaminoglycans influence the glucuronyltransferase-I activity involved in the linkage region synthesis.

The Journal of Biological Chemistry
Yuko ToneKazuyuki Sugahara

Abstract

Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Glucuronyltransferase-I (GlcAT-I) is the key enzyme that completes the synthesis of this linkage region, which is a prerequisite for the conversion of core proteins to functional proteoglycans bearing GAGs. The Xyl and Gal residues in the linkage region can be modified by phosphorylation and sulfation, respectively, although the biological significance of these modifications remains to be clarified. Here we present evidence that these modifications can significantly influence the catalytic activity of GlcAT-I. Enzyme assays showed that the synthetic substrates, Gal-Gal-Xyl(2-O-phosphate)-O-Ser and Gal-Gal(6-O-sulfate)-Xyl(2-O-phosphate)-O-Ser, served as better substrates than the unmodified compound, whereas Gal(6-O-sulfate)-Gal-Xyl(2-O-phosphate)-O-Ser exhibited no acceptor activity. The crystal structure of the catalytic domain of GlcAT-I with UDP and Gal-Gal(6-O-sulfate)-Xyl(2-O-phosphate)-O-S...Continue Reading

References

Sep 11, 1990·Nucleic Acids Research·S Mizushima, S Nagata
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
May 15, 1986·The Biochemical Journal·A R Poole
Oct 1, 1993·Current Opinion in Neurobiology·A D Lander
Sep 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M SalmivirtaU Lindahl
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Jan 21, 2000·Archives of Biochemistry and Biophysics·A OohiraS Aono
Jan 29, 2000·The Journal of Cell Biology·A D Lander, S B Selleck
Aug 18, 2000·The Journal of Biological Chemistry·L C PedersenM Negishi
Oct 24, 2000·Current Opinion in Structural Biology·K Sugahara, H Kitagawa
Aug 22, 2001·The Journal of Biological Chemistry·H KitagawaK Sugahara
Nov 21, 2002·Progress in Brain Research·Daniel A MorgensternJames W Fawcett
Aug 9, 2003·The Journal of Biological Chemistry·Marta Busse, Marion Kusche-Gullberg
Oct 22, 2003·Current Opinion in Structural Biology·Kazuyuki SugaharaHiroshi Kitagawa
Oct 12, 2007·Current Opinion in Structural Biology·Kazuyuki Sugahara, Tadahisa Mikami
Jan 1, 1997·Methods in Enzymology·E A Merritt, D J Bacon
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯

Citations

Nov 21, 2013·Analytical and Bioanalytical Chemistry·Anna AlekseevaAnnamaria Naggi
Sep 17, 2009·Molecular BioSystems·Richard D Cummings
Sep 4, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Gunnar DickKristian Prydz
Oct 9, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Johan Kreuger, Lena Kjellén
Aug 16, 2014·BioMed Research International·Shuji MizumotoKazuyuki Sugahara
Oct 22, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jianzhong WenJack E Dixon
Aug 27, 2015·Biomolecules·Kristian Prydz
Dec 15, 2012·Connective Tissue Research·Casey FrankenbergerTheodore R Oegema
Jan 2, 2013·Trends in Biochemical Sciences·Vincent S TagliabracciJack E Dixon
May 29, 2009·The Biochemical Journal·Toshiyasu KoikeHiroshi Kitagawa
Jun 19, 2013·Biochimica Et Biophysica Acta·Tadahisa Mikami, Hiroshi Kitagawa
Apr 13, 2010·Journal of Molecular Biology·Kosuke HashimotoAnna R Panchenko
Jul 24, 2012·Current Opinion in Structural Biology·Christelle BretonMonica M Palcic
Nov 15, 2008·Biochimica Et Biophysica Acta·Thierry Hennet
Oct 7, 2016·Glycoconjugate Journal·Tadahisa Mikami, Hiroshi Kitagawa
Sep 12, 2018·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Maarten Swart, Linda Troeberg
Jan 24, 2012·International Journal of Experimental Pathology·Bruce Caterson
Sep 22, 2017·Cellular and Molecular Life Sciences : CMLS·Deborah HarrusTuomo Glumoff
Sep 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thibault AnnavalHugues Lortat-Jacob
Aug 15, 2018·Nature Methods·Yen-Hsi ChenZhang Yang
Jun 15, 2019·Orphanet Journal of Rare Diseases·Marlies ColmanFransiska Malfait
Jul 14, 2011·Chemical Reviews·Ryan M SchmaltzChi-Huey Wong
Dec 14, 2021·Frontiers in Cell and Developmental Biology·Shuji Mizumoto, Shuhei Yamada

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.