PMID: 9171399Jun 1, 1997Paper

2-oxo-1,2-dihydroquinoline 8-monooxygenase: phylogenetic relationship to other multicomponent nonheme iron oxygenases

Journal of Bacteriology
B RoscheS Fetzner

Abstract

2-Oxo-1,2-dihydroquinoline 8-monooxygenase, an enzyme involved in quinoline degradation by Pseudomonas putida 86, had been identified as a class IB two-component nonheme iron oxygenase based on its biochemical and biophysical properties (B. Rosche, B. Tshisuaka, S. Fetzner, and F. Lingens, J. Biol. Chem. 270:17836-17842, 1995). The genes oxoR and oxoO, encoding the reductase and the oxygenase components of the enzyme, were sequenced and analyzed. oxoR was localized approximately 15 kb downstream of oxoO. Expression of both genes was detected in a recombinant Pseudomonas strain. In the deduced amino acid sequence of the NADH:(acceptor) reductase component (OxoR, 342 amino acids), putative binding sites for a chloroplast-type [2Fe-2S] center, for flavin adenine dinucleotide, and for NAD were identified. The arrangement of these cofactor binding sites is conserved in all known class IB reductases. A dendrogram of reductases confirmed the similarity of OxoR to other class IB reductases. The oxygenase component (OxoO, 446 amino acids) harbors the conserved amino acid motifs proposed to bind the Rieske-type [2Fe-2S] cluster and the mononuclear iron. In contrast to known class IB oxygenase components, which are composed of differing s...Continue Reading

References

Jan 1, 1992·Annual Review of Microbiology·J R Mason, R Cammack
Jan 1, 1992·Annual Review of Microbiology·S HarayamaE L Neidle
Jun 15, 1989·Biochemical and Biophysical Research Communications·S FetznerF Lingens
Oct 1, 1988·Journal of Bacteriology·F Brunel, J Davison
Oct 14, 1970·Journal of Molecular Biology·M Mandel, A Higa
Jul 28, 1995·The Journal of Biological Chemistry·B RoscheF Lingens

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Citations

Sep 25, 1998·The Journal of Biological Chemistry·A FerrándezE Díaz
Apr 17, 2001·Bioscience, Biotechnology, and Biochemistry·J W NamT Omori
Oct 11, 2003·FEMS Microbiology Reviews·Joseph G LeahySuzanne M Morcomb
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·E DíazJ L García
May 8, 2001·Archives of Biochemistry and Biophysics·C C Marohnic, M J Barber
Sep 22, 2001·Archives of Biochemistry and Biophysics·M J BarberC C Marohnic

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