PMID: 6989358Jan 15, 1980Paper

2-oxocarboxylic acids and function of pancreatic islets in obese-hyperglycaemic mice. Insulin secretion in relation to 45Ca uptake and metabolism

The Biochemical Journal
S Lenzen, U Panten

Abstract

The effects of aliphatic 2-oxocarboxylic acids, at concentrations of up to 40mm, on the function of pancreatic islets from ob/ob (obese-hyperglycaemic) mice were investigated. 1. 2-Oxopentanoate, dl-3-methyl-2-oxopentanoate, 4-methyl-2-oxopentanoate and 2-oxohexanoate all induced insulin release by isolated incubated islets and a biphasic insulin-secretory pattern in perfused mouse pancreas. The last two substances were similar in potency to glucose. Pyruvate, 2-oxobutyrate, 3-methyl-2-oxobutyrate and 2-oxo-octanoate did not induce insulin release significantly. 2. 2-Oxocarboxylic acids with significant insulin-secretory potency also induced significant (45)Ca uptake by isolated incubated islets. 3. The rates of decarboxylation of [1-(14)C]pyruvate, 3-methyl-2-oxo[1-(14)C]butyrate and 4-methyl-2-oxo[1-(14)C]pentanoate were twice as high as the rates of oxidation of the corresponding U-(14)C-labelled compounds. However, whereas the rates of metabolism of labelled pyruvate and 3-methyl-2-oxobutyrate steadily increased over the concentration range 1-40mm, those of labelled 4-methyl-2-oxopentanoate and d-[U-(14)C]glucose levelled off at concentrations above 10mm. 4. Omission of (40)CaCl(2) from the incubation medium reduced the rat...Continue Reading

Citations

Oct 15, 1984·Experientia·A Sener, W J Malaisse
Oct 1, 1988·Scandinavian Journal of Clinical and Laboratory Investigation·G Schäfer, P Schauder
Jan 1, 1986·Diabetes/metabolism Reviews·M D Meglasson, F M Matschinsky
Jul 6, 2006·The Biochemical Journal·Inés Hernández-FisacJorge Tamarit-Rodriguez
May 17, 2012·The Biochemical Journal·Katarzyna HanzelkaEwa Gurgul-Convey
Jan 29, 2009·The Biochemical Journal·Javier Pizarro-DelgadoJorge Tamarit-Rodriguez

Related Concepts

Calcium
Dose-Response Relationship, Drug
Saturated Fat
Glucose, (beta-D)-Isomer
Novolin
Islets of Langerhans
Keto Acids
Mouse, Hyperglycemic
Perfusion
Secretory Rate

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