PMID: 2493071Mar 1, 1989Paper

2-Oxoglutarate: oxidation and role as a potential precursor of cytosolic acetyl-CoA for the synthesis of acetylcholine in rat brain synaptosomes

Journal of Neurochemistry
J WilloughbyJ B Clark

Abstract

The possibility that 2-oxoglutarate may supply acetyl units for the cytosolic synthesis of acetylcholine in rat brain synaptosomes was investigated. The contribution of [14C]2-oxoglutarate to the synaptosomal synthesis of [14C]acetylcholine was found to be negligible despite evidence for its uptake and oxidation. The activity of the enzymes NADP-isocitrate dehydrogenase (EC 1.1.1.42), aconitate hydratase (EC 4.2.1.3), and ATP citrate-lyase (EC 4.1.3.8) were measured in the synaptosol. NADP-isocitrate dehydrogenase and aconitate hydratase are present at three- to 1.5-fold higher activities than ATP citrate-lyase. It seems likely that these enzymes contribute to the metabolism of citrate and prevent detectable formation of cytosolic acetyl-CoA from exogenously added 2-oxoglutarate (or citrate). The data further suggest that ATP citrate-lyase may in part be associated with the mitochondrial fraction.

References

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Citations

Sep 1, 1993·Neurochemistry International·A M CiminiM P Cerú
Dec 3, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Laszlo Tretter, Vera Adam-Vizi
May 20, 1990·European Journal of Biochemistry·W BloisiS Didonato

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