PMID: 11325257Apr 28, 2001Paper

2'-Ribose-ferrocene oligonucleotides for electronic detection of nucleic acids

The Journal of Organic Chemistry
C J YuY Chong

Abstract

We have synthesized two novel phosphoramidites with a ferrocenyl moiety at the 2'-ribose position linked through a butoxy linker. Using automated DNA/RNA synthesis techniques, oligonucleotides containing ferrocene at various positions were prepared and characterized by HPLC, MALDI-TOF mass spectrometry, and electrochemistry. Thermal stability studies of the ferrocene-modified DNA duplexes revealed that introduction of one or two ferrocenyl complexes does not result in an observed change of the T(m) values of the corresponding DNA duplexes when compared to the nonmodified hybrids. These data indicate that the introduction of a ferrocenyl group at the 2'-position of the ribose ring containing either a purine or pyrimidine base has no effect on the stability of the modified DNA. The electrochemical behavior of the ferrocene-containing DNA was examined by cyclic voltammetry. The modified 2'-ferrocene-oligonucleotides are electrochemically active and can be used as signaling probes for the electronic detection of nucleic acids on bioelectronic sensors.

Citations

Nov 20, 2004·Chemistry : a European Journal·Andrew R PikeAndrew Houlton
Mar 17, 2004·Biosensors & Bioelectronics·Wei CaiRobert J Hamers
Jan 23, 2010·Journal of the American Chemical Society·Rajesh SardarRoyce W Murray
Oct 7, 2004·Nucleic Acids Research·Aude-Emmanuelle NavarroHugues Brisset
Jun 13, 2002·Nucleic Acids Research·Wjatschesslaw A Wlassoff, Garry C King
Jan 11, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kosuke MukumotoShigeori Takenaka
Dec 20, 2005·Bioorganic & Medicinal Chemistry Letters·Martine FayolleAsha Parbhu Patel
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