20-HETE downregulates Na/K-ATPase α1 expression via the ubiquitination pathway.

Prostaglandins & Other Lipid Mediators
Bijun ZhangYanyan Zhao

Abstract

In this article, we found that 20-Hydroxyeicosatetraenoic acid (20-HETE) reduced Na/K-ATPase α1 expression via the ubiquitin-proteasome pathway. The ubiquitination level of Na/K-ATPase α1 protein was increased in 20-HETE-treated mouse cortical collecting duct cells and the kidney tissues of CYP4F2 transgenic mice. We also demonstrated that 20-HETE-induced high level phosphorylation of Na/K-ATPase α1 was necessary for its ubiquitination.The protein kinase C inhibitor sotrastaurin significantly reduced the phosphorylation of Na/K-ATPase α1 and increased the expression of Na/K-ATPase α1 although 20-HETE stimulus being applied at the same time. Moreover, high level of 20-HETE increased the expression and neddylation of Cullin3,which is an important ubiquitin E3 ligase in kidney. MLN4924, an inhibitor of NEDD8-activating enzyme, inhibited neddylation of Cullin3 and reversed the reduction of Na/K-ATPase α1 expression caused by 20-HETE. Thus, 20-HETE downregulates Na/K-ATPase α1 via the ubiquitination pathway, and phosphorylation of Na/K-ATPase α1 is a prerequisite to ubiquitination. Additionally, 20-HETE regulates Cullin3 expression via neddylation.

References

Dec 16, 1997·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·J A Tumlin
Jun 8, 2004·Current Drug Metabolism·A Sarkis, R J Roman
Apr 22, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sijun ZhuTzumin Lee
Apr 19, 2007·The Journal of Biological Chemistry·Eng-Hui Chew, Thilo Hagen
May 29, 2007·Cellular Signalling·Laura A DadaJacob I Sznajder
Sep 25, 2014·The Journal of Clinical Investigation·James A McCormickDavid H Ellison
Oct 15, 2014·Biomolecules·Cathal MahonElah Pick
Nov 27, 2015·Biochemical and Biophysical Research Communications·Chong ZhangChao-Ling Yang
Apr 15, 2016·American Journal of Physiology. Renal Physiology·Mohammed Z Ferdaus, James A McCormick
Jun 12, 2016·Prostaglandins & Other Lipid Mediators·Maayan WaldmanEdith Hochhauser
Sep 14, 2019·International Journal of Molecular Sciences·Shashank ShekharFan Fan
Mar 17, 2020·Experimental Cell Research·Jianqiao TuYanyan Zhao

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