PMID: 6401862Jan 1, 1983Paper

20-Hydroxyecdysone stimulates the accumulation of translatable yolk polypeptide gene transcript in adult male Drosophila melanogaster

Proceedings of the National Academy of Sciences of the United States of America
P D ShirkJ H Postlethwait

Abstract

Yolk polypeptide (YP) synthesis is hormonally stimulated during maturation of adult female Drosophila melanogaster. Synthesis of the three YPs is sex specific and occurs in fat body cells and follicle cells of adult females. However, males have been shown to produce YPs when treated with the steroid hormone 20-hydroxyecdysone (20-HE). By using a cell-free translation system as an assay for YP mRNA, we found that 20-HE also causes the accumulation of translatable YP message in males. In addition, hybridization of cloned copies of genes for both YP1 and YP3 to total RNA from males showed that 20-HE caused the appearance of YP gene transcripts in males. Eight hours after treatment of males with 20-HE, YP gene transcript levels had increased at least 25-fold to approximately 2.7 x 10(6) copies of YP1 gene transcript per adult male fly. In normal adult females, there were 42 x 10(6) copies per fly by 24 hr. There was neither detectable YP synthesis nor translatable YP gene transcript in either normal 1- to 3-day-old males or 24-hr-old males treated with a juvenile hormone analogue. This evidence shows that 20-HE acts to regulate the levels of translatable YP mRNA in male Drosophila.

References

Dec 20, 1979·Nucleic Acids Research·J P GergenP C Wensink
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J K Skipper, T H Hamilton
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A T BurnsR F Goldberger
Apr 1, 1977·The Journal of Experimental Zoology·M Bownes, B D Hames
Oct 1, 1977·Developmental Biology·R B HodgettsJ D O'Connor
Dec 1, 1977·The Journal of Experimental Zoology·A M Handler, J H Postlethwait
Feb 1, 1978·The Journal of Experimental Zoology·J H Postlethwait, G J Jones
Sep 3, 1976·Science·H Weintraub, M Groudine
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Jan 1, 1982·Developmental Biology·M D BrennanA P Mahowald
Oct 1, 1980·Developmental Biology·J H PostlethwaitT Jowett
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G P KraminskyR B Hodgetts

❮ Previous
Next ❯

Citations

Dec 1, 1985·Biochemical Genetics·P Minoo, J H Postlethwait
Nov 1, 1988·Developmental Biology·J SpiethT Blumenthal
May 29, 2003·Journal of Insect Physiology·M F. WolfnerL A. Herndon
Apr 16, 1998·Insect Biochemistry and Molecular Biology·G Don-Wheeler, F Engelmann
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T TamuraJ Postlethwait
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P CherbasK Nakanishi
Jun 14, 2008·BMC Developmental Biology·Piotr BebasJadwiga M Giebultowicz
Feb 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·V K WalkerW G Bendena
May 1, 1989·Molecular and Cellular Endocrinology·A M Handler, P Maroy
Feb 19, 2009·Journal of Insect Physiology·Joanna KotwicaJadwiga M Giebultowicz
Jan 1, 1990·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·M J Birnbaum, L I Gilbert
Jan 1, 1997·Archives of Insect Biochemistry and Physiology·A M Handler

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.