20-OH-ecdysone regulates 60 C beta tubulin gene expression in Kc cells and during Drosophila development

Experimental Cell Research
M L SobrierB Dastugue

Abstract

Cultured Kc cells of Drosophila melanogaster are sensitive to the insect moulting hormone, 20-hydroxy-ecdysone (20-OH-E). Morphological changes of Kc-treated cells were observed and electron microscopic analysis of pseudopodia shows a large increase in the number of microtubules, all arranged in the same orientation. The 60 C beta tubulin gene which is expressed only in 20-OH-E-treated cells encodes a 2.6-kb mRNA which is essentially cytoplasmic and polyadenylated. The corresponding premessenger is 7 kb in length and is absent in untreated cells. Two peaks of expression of the 60 C beta tubulin gene are observed during Drosophila development: at midembryogenesis (stage 8-13 h) and at the late third instar larvae-early pupae stage. By use of the Ecdysone 1 mutant, 60 C beta tubulin gene expression was demonstrated to be regulated in part by 20-OH-E during Drosophila development. Through these two complementary biological models of study, the mode and role of beta tubulin gene regulation are discussed.

References

Jan 25, 1988·Biochimica Et Biophysica Acta·P MontpiedB Dastugue
Nov 25, 1986·Nucleic Acids Research·F PeronnetM Best-Belpomme
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A GarenJ A Lepesant

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