20S proteasome from LMP7 knock out mice reveals altered proteolytic activities and cleavage site preferences

FEBS Letters
R StohwasserP M Kloetzel

Abstract

20S proteasomes of tissues from LMP7 knock out mice which show reduced MHC class I restricted antigen presentation were analyzed with regard to their subunit composition, peptide hydrolyzing activity and their ability to cleave a synthetic 25-mer polypeptide. LMP7 deficiency results in an enhanced incorporation of subunit MB1 and in a 2-3.8-fold increase in Vmax for the Suc-LLVY-MCA hydrolyzing activity. Since LMP7 deficiency also affects the cleavage site preference of 20S proteasomes the reduced MHC class I antigen presentation of LMP7 knock out mice is most likely due to an impairment in peptide generation.

References

Jan 15, 1992·European Journal of Biochemistry·A L Goldberg
Jan 1, 1993·Enzyme & Protein·M GaczynskaA L Goldberg
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·V UstrellM Rechsteiner
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·M GaczynskaA L Goldberg
Aug 26, 1994·Science·H J FehlingH von Boehmer

❮ Previous
Next ❯

Citations

Nov 28, 2000·International Journal of Cancer. Journal International Du Cancer·T FrisanM G Masucci
Oct 4, 2000·Journal of Peptide Science : an Official Publication of the European Peptide Society·A J Rivett, R C Gardner
Aug 30, 2002·Molecular Immunology·Monique Grommé, Jacques Neefjes
Aug 1, 1996·Current Opinion in Biotechnology·D StockJ Löwe
Jan 31, 1998·The Journal of Experimental Medicine·T A GriffinR A Colbert
Jan 13, 2000·Immunological Reviews·I A YorkK L Rock
Jun 8, 1999·Annual Review of Immunology·K L Rock, A L Goldberg
Sep 1, 1996·Biological Chemistry·R Stohwasser, P M Kloetzel
Mar 21, 2008·Molecular BioSystems·Marie WehenkelKyung Bo Kim
Jan 3, 2016·Archives of Biochemistry and Biophysics·Burkhardt Dahlmann
Nov 5, 2013·Rheumatic Diseases Clinics of North America·Gina A Montealegre SanchezRaphaela Goldbach-Mansky
Oct 16, 2015·European Journal of Immunology·Sarah MundtMichael Basler
May 8, 2007·Experimental Neurology·Krishna PuttaparthiJeffrey L Elliott
Mar 30, 2010·Journal of Neurochemistry·Stacy A HussongDeborah A Ferrington
Feb 25, 2000·Protein Expression and Purification·A M EleuteriE Fioretti
Apr 29, 2015·Analytical Biochemistry·Nathalie VigneronBenoît J Van den Eynde
Feb 24, 2015·Annual Review of Immunology·Adriana Almeida de JesusRaphaela Goldbach-Mansky
Nov 5, 1997·The Journal of Biological Chemistry·C RealiniM Rechsteiner
Mar 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christy M CaudillThomas A Griffin
May 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael BaslerJohn J Monaco
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alexander K NussbaumJ Lindsay Whitton
Aug 29, 2019·Frontiers in Oncology·Alexey V Morozov, Vadim L Karpov
May 31, 2019·Inflammation and Regeneration·Nobuo KanazawaMegumu K Saito
Mar 13, 2021·Cancer Immunology Research·Hanna LeisterAlexander Visekruna
Apr 25, 2000·The Journal of Laboratory and Clinical Medicine·J E NelsonC Cardozo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.