21st Century Genetics: Mass Spectrometry of Yeast Telomerase

Cold Spring Harbor Symposia on Quantitative Biology
Kah-Wai Lin, Virginia A Zakian

Abstract

Telomerase is a specialized reverse transcriptase that maintains the ends of chromosomes in almost all eukaryotes. The core of telomerase consists of telomerase RNA and the reverse transcriptase that uses a short segment without the RNA to template the addition of telomeric repeats. In addition, one or more accessory proteins are required for telomerase action in vivo. The best-studied accessory protein is Est1, which is conserved from yeasts to humans. In budding yeast, Est1 has two critical in vivo functions: By interaction with Cdc13, a telomere-binding protein, it recruits telomerase to telomeres, and it also increases telomerase activity. Although budding yeast telomerase is highly regulated by the cell cycle, Est1 is the only telomerase subunit whose abundance is cell cycle-regulated. Close to 400 yeast genes are reported to affect telomere length, although the specific function of most of them is unknown. With the goal of identifying novel telomerase regulators by mass spectrometry, we developed methods for purifying yeast telomerase and its associated proteins. We summarize the methods we used and describe the experiments that show that four telomerase-associated proteins identified by mass spectrometry, none of which h...Continue Reading

References

Oct 21, 1994·Science·M S Singer, D E Gottschling
May 3, 1996·Cell·R J WellingerV A Zakian
Feb 25, 2000·Molecular and Cellular Biology·J ZhouB Futcher
May 10, 2000·Current Biology : CB·S MarcandE Gilson
Aug 10, 2002·Science·Andrew K P TaggartVirginia A Zakian
Nov 5, 2002·Genes & Development·Anita G SetoThomas R Cech
May 8, 2004·Genes & Development·Keren L Witkin, Kathleen Collins
May 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Syed H AskreeMichael J McEachern
Jun 17, 2004·Genes & Development·Michel LarrivéeRaymund J Wellinger
Jul 20, 2004·EMBO Reports·Christian SchuberthAlexander Buchberger
Nov 9, 2004·Nature Structural & Molecular Biology·Timothy S FisherVirginia A Zakian
Aug 27, 2005·Nature·Jean-Baptiste BouléVirginia A Zakian
Sep 13, 2005·Molecular & Cellular Proteomics : MCP·Ileana M CristeaMichael P Rout
Jul 25, 2006·Nature Structural & Molecular Biology·Jennifer L OsterhageKatherine L Friedman
Oct 5, 2006·Molecular Cell·Momchil D Vodenicharov, Raymund J Wellinger
Oct 31, 2006·Molecular Cell·Christopher J FrankCarol W Greider
Dec 13, 2006·The Journal of Biological Chemistry·Stéphanie LaroseSherif Abou Elela
Jan 16, 2007·Molecular and Cellular Biology·Keren L WitkinKathleen Collins
Jul 28, 2007·Molecular Cell·Michelle SabourinVirginia A Zakian
Aug 22, 2008·Nature·Pablo HuertasStephen P Jackson
Jan 28, 2009·Nature Structural & Molecular Biology·Eun Young YuNeal F Lue
Jan 28, 2009·Nature Structural & Molecular Biology·Jaesung LeeVictoria Lundblad
Apr 24, 2009·Nucleic Acids Research·Lior UngarMartin Kupiec
Jul 15, 2009·Molecular Cell·Diego BonettiMaria Pia Longhese
Nov 9, 2010·Nature Structural & Molecular Biology·Jean S McGeeVirginia A Zakian
Dec 7, 2010·Nature Methods·Hyungwon ChoiAlexey I Nesvizhskii
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yun Wu, Virginia A Zakian
Aug 24, 2013·Nature Reviews. Immunology·Peter D KwongGary J Nabel
Mar 13, 2014·Molecular Cell·Ada NdojaTingting Yao
Mar 25, 2014·Molecular Cell·Julien SoudetMaria Teresa Teixeira

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Citations

Mar 17, 2017·Annual Review of Biophysics·Henry ChanJuli Feigon
Feb 1, 2017·Annual Review of Biochemistry·R Alex WuKathleen Collins

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