2,3-Dihydroxyisovalerate production by Klebsiella pneumoniae.

Applied Microbiology and Biotechnology
Yike WangJian Hao

Abstract

2,3-Dihydroxyisovalerate is an intermediate of valine and leucine biosynthesis pathway; however, no natural microorganism has been found yet that can accumulate this compound. Klebsiella pneumoniae is a useful bacterium that can be used as a workhorse for the production of a range of industrially desirable chemicals. Dihydroxy acid dehydratase, encoded by the ilvD gene, catalyzes the reaction of 2-ketoisovalerate formation from 2,3-dihydroxyisovalerate. In this study, an ilvD disrupted strain was constructed which resulted in the inability to synthesize 2-ketoisovalerate, yet accumulate 2,3-dihydroxyisovalerate in its culture broth. 2,3-Butanediol is the main metabolite of K. pneumoniae and its synthesis pathway and the branched-chain amino acid synthesis pathway share the same step of the α-acetolactate synthesis. By knocking out the budA gene, carbon flow into the branched-chain amino acid synthesis pathway was upregulated, which resulted in a distinct increase in 2,3-dihydroxyisovalerate levels. Lactic acid was identified as a by-product of the process and by blocking the lactic acid synthesis pathway, a further increase in 2,3-dihydroxyisovalerate levels was obtained. The culture parameters of 2,3-dihydroxyisovalerate ferme...Continue Reading

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Citations

Sep 12, 2020·Enzyme and Microbial Technology·Yang YangJian Hao
Nov 9, 2021·Applied Microbiology and Biotechnology·Shaoqi SunJian Hao

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Methods Mentioned

BETA
PCR
nuclear magnetic resonance
ion-exchange chromatography

Software Mentioned

ChemDraw

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