2,3,7,8-Tetrachlorodibenzo-p-dioxin induced cytochrome P450s alter the formation of reactive oxygen species in liver cells

Molecular Nutrition & Food Research
Stefanie KnerrD Schrenk

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) was classified by the International Agency for Research on Cancer as a carcinogen in humans. It acts through an aryl hydrocarbon receptor-mediated mechanism, inducing the transcription of numerous genes, including various cytochrome P450s (CYPs - CYP1A1, 1A2, 1B1). Induction of CYPs may lead to genotoxicity by generating reactive oxygen species (ROS) which can damage DNA directly and/or via the generation of reactive metabolites. We determined ROS formation with the 2',7'-dihydrodichlorofluorescein diacetate fluorescence assay after incubation of HepG2 hepatoma cells or primary rat hepatocytes with TCDD. The amount of 8-oxo-2'-deoxyguanosine (8-oxo-dG) in DNA was measured using HPLC-MS/MS, the amount of CYP1A1 protein by Western blotting. The catalytic activity of CYP1A enzymes was determined as 7-ethoxyresorufin-O-deethylase (EROD) activity. Incubation of cells with TCDD for 48 h caused increased levels of ROS in primary rat hepatocytes as well as increased levels of 8-oxo-dG in DNA compared to untreated cells. In the HepG2 cell line no significant effects were observed for both ROS formation and 8-oxo-dG levels. Both effects were in good agreement with the extent of induction of CYP1...Continue Reading

References

Nov 1, 1978·Toxicology and Applied Pharmacology·R J KocibaC G Humiston
Sep 1, 1982·European Journal of Biochemistry·H Kuthan, V Ullrich
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·J Y ParkB N Ames
Jan 1, 1996·Annual Review of Cell and Developmental Biology·J V Schmidt, C A Bradfield
Jan 9, 1998·Drug Metabolism Reviews·J P WhitlockR Clarke-Katzenberg
May 20, 1999·Annual Review of Pharmacology and Toxicology·J P Whitlock
Sep 6, 2002·Chemico-biological Interactions·Mark E Hahn
Sep 4, 2004·Environmental Health Perspectives·Kyle SteenlandManolis Kogevinas
Oct 9, 2004·Molecular Interventions·Jin Mo Chung

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Citations

Mar 7, 2007·Archives of Toxicology·Yasuaki DewaKunitoshi Mitsumori
Aug 30, 2008·Archives of Toxicology·Yasuaki DewaKunitoshi Mitsumori
Apr 18, 2009·Archives of Environmental Contamination and Toxicology·Chun-Feng LuJing Zhao
Sep 17, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Toufan ParmanCarol E Green
Nov 13, 2014·Environmental Science and Pollution Research International·Ilavarasi KalaiselvanPandima Devi Kasi
May 6, 2009·Toxicology Letters·Luisa MartinoVincenzo De Tata
Jul 7, 2015·Regulatory Toxicology and Pharmacology : RTP·Richard A BeckerRobert A Budinsky
Feb 3, 2009·The Journal of Toxicological Sciences·Sayaka MatsumotoKunitoshi Mitsumori
Jun 27, 2014·Environmental Research·Diego BadernaEmilio Benfenati
Jan 15, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Xiaoyan LuXiaohui Fan
Jul 25, 2012·The Science of the Total Environment·Maria Stefania SpagnuoloLeopoldo Iannuzzi
Nov 11, 2016·Stem Cells International·Cornelia Dietrich
Dec 28, 2012·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Hyun-Sook KimWoon-Won Jung
Sep 16, 2006·Molecular Nutrition & Food Research·Stefanie Knerr, Dieter Schrenk
Sep 4, 2020·Biological & Pharmaceutical Bulletin·Min Cheol PyoKwang-Won Lee
Jan 11, 2020·Environmental Science and Pollution Research International·Romain ColpaertXavier Moreau

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