2,3,7,8-Tetrachlorodibenzo-p-dioxin induces a proteomic pattern that defines cleft palate formation in mice

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Xingang YuanYuexian Fu

Abstract

To identify the differential protein pattern in 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induced cleft palates using a proteomic approach. At gestation day (GD) 12, TCDD (64 g/kg; n = 30) or corn oil control (n = 30) was given to time-pregnant C57BL/6J mice by gavage. The anatomical, histological, proteomic changes in the palates of the fetal mice were studied on GD18. Total protein was extracted from the palate tissue and examined by 2-dimensional gel electrophoresis (2-DE). Spots differentially expressed between the two groups were selected for analysis by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). The proteins were identified by data searching in the Mascot database. In TCDD group, the incidence of cleft palate was 100%. Ten differential protein spots with the largest fold change were selected for further identification by mass spectrometry, 7 showed significantly higher volumes and 3 showed significantly lower volumes in TCDD palates than the control palates (all p<0.05). Peroxiredoxin-1 were robustly up-regulated in the cleft palate group, as well proteins linked to energy metabolism, cell migration, and apoptosis. Peroxiredoxin-1 protein may be associated with cleft pala...Continue Reading

References

Nov 1, 1971·Toxicology and Applied Pharmacology·K D Courtney, J A Moore
Oct 6, 1997·Genes & Development·L Van Aelst, C D'Souza-Schorey
Feb 27, 1999·Toxicological Sciences : an Official Journal of the Society of Toxicology·B D AbbottJ Schmid
Apr 6, 1999·Trends in Biotechnology·W P Blackstock, M P Weir
Apr 1, 2000·Science·R F Service
Mar 23, 2002·Cadernos de saúde pública·Isabel Cristina Gonçalves LeiteSérgio Koifman
May 29, 2002·Clinical Genetics·J C Murray
Jan 14, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Beth A VorderstrasseB Paige Lawrence
Jun 30, 2004·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·J LittleUNKNOWN United Kingdom-based case-control study
May 14, 2005·Antioxidants & Redox Signaling·Stephan Immenschuh, Eveline Baumgart-Vogt
Jan 24, 2006·Cellular and Molecular Life Sciences : CMLS·S Saussez, R Kiss
May 11, 2006·Drug Metabolism Reviews·Heather M H Goldstone, John J Stegeman

❮ Previous
Next ❯

Citations

Oct 28, 2015·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·M C Buser, H R Pohl
May 16, 2014·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·I MillerA C Gutleb

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.