2,3,7,8-Tetrachlorodibenzo-p-dioxin induces apoptotic cell death and cytochrome P4501A expression in developing Fundulus heteroclitus embryos

Aquatic Toxicology
B H ToomeyR T Di Giulio

Abstract

Fundulus heteroclitus embryos were exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) during early development using nanoinjection or water bath exposure. TCDD caused developmental abnormalities that included hemorrhaging, loss of vascular integrity, edema, stunted development and death. The LC(50) and LD(50) of TCDD for Fundulus embryos were approximately 19.7+/-9.5 pg TCDD/microl (water bath) and 0.25+/-0.09 ng TCDD/g embryo (nanoinjection). To identify a possible cause for these developmental abnormalities we analyzed the effects of TCDD on apoptotic cell death and cytochrome P4501A (CYP1A) expression in the embryos. TCDD exposure increased apoptotic cell death in several tissues including brain, eye, gill, kidney, tail, intestine, heart, and vascular tissue. CYP1A expression was also increased in the TCDD-exposed embryos predominantly in liver, kidney, gill, heart, intestine, and in vascular tissues throughout the embryo. There was co-occurrence of TCDD-induced apoptosis and CYP1A expression in some, but not all, cell types. In addition the dose response relationships for apoptosis and mortality were similar, while CYP1A expression appeared more sensitive to TCDD induction.

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Citations

Oct 12, 2012·Environmental Science and Pollution Research International·Jon A DoeringMarkus Hecker
Jul 25, 2003·Toxicology and Applied Pharmacology·Rachel M PattersonDori Germolec
Dec 25, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Sonya M BilliardRichard T Di Giulio
Jun 25, 2014·Environmental Science and Pollution Research International·Sabrina SchiwySteffen H Keiter
Oct 1, 2011·Molecular and Cellular Endocrinology·Tisha C King-HeidenRichard E Peterson
Aug 21, 2013·Saudi Journal of Biological Sciences·Mohammad Niamat AliWaseem Ahmad
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May 12, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Sonya M BilliardRichard T Di Giulio
Aug 2, 2003·Congenital Anomalies·Hiroki TeraokaTakeo Hiraga
Feb 11, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·David C VolzSeth W Kullman
Mar 24, 2004·The Journal of Experimental Biology·Yu-Wen Chung-DavidsonWeiming Li
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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis