Abstract
n-Hexane is an organic solvent widely used in industry. 2,5-Hexanedione (2,5-HD), the major neurotoxic metabolite of n-hexane, decreases the levels of neurofilaments (NFs) in neurons. Neurogenesis occurs throughout life, and the hippocampal dentate gyrus is one of two major brain areas showing neurogenesis in adulthood. In the current study, rats were intraperitoneally injected with normal saline solution or 2,5-HD five times per week for five continuous weeks. 2,5-HD was administered to the low-dose and high-dose groups at 200 and 400 mg/kg/day, respectively. Then, immunoreactive cells were counted in the hippocampal granule cell layer (GCL) and subgranular zone (SGZ). Ki67+ cells significantly decreased in the high-dose group, while the percentage of proliferative Sox2+ cells significantly increased, consistent with high hippocampal Sox2 expression. Additionally, western blotting showed that exposure to high doses of 2,5-HD led to decreased NF-L in both the cortex and hippocampus, whereas low doses led to a significant reduction in the cortex only. In conclusion, 2,5-HD increases the percentage of proliferating neural stem and progenitor (Sox2+) cells in the SGZ/GCL.
References
Dec 17, 1997·Experimental Neurology·Q ZhuJ P Julien
Mar 1, 2002·Toxicology Letters·R CarneyM J Rowan
Sep 2, 2003·Neuron·Victoria GrahamLarysa Pevny
Jun 23, 2004·Toxicology and Applied Pharmacology·Richard M LoPachinLisa A Opanashuk
Feb 17, 2005·Neurotoxicology·Richard M LoPachinMaria L Reid
Sep 22, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Robert E IosifOlle Lindvall
Apr 21, 2007·Neurochemical Research·Fuyong SongKeqin Xie
Jun 21, 2007·Current Biology : CB·Stewart Shipp
Nov 16, 2007·Biochemical Pharmacology·Fuyong SongKeqin Xie
Feb 26, 2008·Cell·Chunmei ZhaoFred H Gage
Mar 29, 2008·Cell Stem Cell·Hoonkyo SuhFred H Gage
May 26, 2009·Toxicology·Min-Sun KimJaewon Lee
Sep 8, 2009·Nature Neuroscience·Rebecca FavaroSilvia K Nicolis
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ja Wook KooRonald S Duman
May 26, 2011·Neuron·Guo-Li Ming, Hongjun Song
Jul 1, 2005·Environmental Toxicology and Pharmacology·Tian-Liang ZhangKe-Qin Xie
May 9, 2012·Neurotoxicology·Shuang LiuWenchang Sun
Jul 31, 2012·Neurotoxicology·Xin ChengXuesong Yang
Apr 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Alejandro Amador-ArjonaAlexey V Terskikh
Feb 19, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Megan Bowers, Sebastian Jessberger
Citations
Mar 13, 2020·Toxicologic Pathology·Peter S Spencer
Nov 20, 2020·Free Radical Biology & Medicine·Ruixue HuangQingshan Wang
Aug 30, 2020·Chemico-biological Interactions·Ruixue HuangQingshan Wang