PMID: 6160465Jul 25, 1980Paper

2'5' oligoadenylate synthetase, an interferon induced enzyme: direct assay methods for the products, 2'5' oligoadenylates and 2'5' co-oligonucleotides

Nucleic Acids Research
J JustesenM N Thang

Abstract

The interferon induced enzyme 2'5' oligoadenylate synthetase produces 2'5' pppA(pA)n the first discovered natural nucleotide with a 2'5' linkage. We describe a direct assay of this enzyme based on separation by thin layer chromatography (TLC) of the substrate ATP and the products 2'5' pppA(pA)n (n larger than or equal to 1). This technique presents obvious advantages compared to the currently used methods. Moreover the enzyme uses other nucleotides as substrates forming co-oligonucleotides 2'5 pppA(pA)n pN (N = U,G,C,dA,dG,dT and dC). Additional procedures are described using different developing solvent systems for the separation of the core-2'5' oligonucleotides (2'5' A(pA)npN) containing AMP-residues entirely and those with another nucleotide at the 2' end.

Citations

Mar 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A PoitrineJ C Chaput
Aug 1, 1986·Journal of Interferon Research·J Justesen, K Berg
Apr 15, 2010·Marine Drugs·Annika LoppMerike Kelve
Jan 1, 1980·Annals of the New York Academy of Sciences·J JutesenM N Thang
Oct 30, 2001·FEBS Letters·R HartmannJ Justesen
Apr 1, 1987·Journal of Interferon Research·J Rovnak, R S Ranu
Jun 27, 2015·BMC Biochemistry·Jesper Buchhave PoulsenPia Møller Martensen
Mar 7, 1998·The Journal of Biological Chemistry·R HartmannJ Justesen
Jun 12, 1998·Pharmacology & Therapeutics·M R Player, P F Torrence
Jun 1, 1981·Microbiological Reviews·J Gordon, M A Minks
Oct 18, 1983·Biochimica Et Biophysica Acta·T J BorelliJ M Wu
Nov 15, 1992·Analytical Biochemistry·J Justesen, N O Kjeldgaard
Nov 30, 1983·Biochemical and Biophysical Research Communications·J M WuA R Semproni
May 29, 1981·Biochemical and Biophysical Research Communications·D FerbusM N Thang

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