2.6 A resolution crystal structure of helices of the motile major sperm protein (MSP) of Caenorhabditis elegans

Journal of Molecular Biology
Anne M E BakerMurray Stewart

Abstract

The amoeboid locomotion of nematode sperm is mediated by the assembly dynamics of the major sperm protein (MSP). MSP forms fibrous networks based on a hierarchy of macromolecular assemblies: helical subfilaments are built from MSP dimers; filaments are formed from two subfilaments coiling round one another; and filaments themselves supercoil to produce bundles. To provide a structural context for understanding the role of these macromolecular assemblies in cell locomotion, we have determined the 2.6 A resolution structure of crystals of Caenorhabditis elegans MSP that are constructed from helices of MSP chains that are analogous to the subfilaments from which filaments are constructed. Comparison with the crystal structures of dimers and helical assemblies of Ascaris suum MSP has identified five conserved interaction interfaces that suggest how subfilaments interact in filaments and how filaments can form bundles. The interfaces frequently involve the loop containing residues 78-85, which is divergent between MSP homologues, and the loop containing residues 98-103, which is highly conserved.

References

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Citations

Apr 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Long MiaoThomas M Roberts
Apr 20, 2005·Trends in Parasitology·Deirdre E K Tarr, Alan L Scott
Nov 21, 2007·Journal of Cellular Biochemistry·Antonio del Castillo-Olivares, Harold E Smith
Dec 25, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Sung Min HanMichael A Miller
Jun 12, 2004·Neurosurgical Focus·Marie Simard
Nov 23, 2017·G3 : Genes - Genomes - Genetics·Katja R Kasimatis, Patrick C Phillips
Sep 16, 2017·Scientific Reports·Wendy ZhangJohnny K Ngsee
Jul 29, 2004·Molecular and Biochemical Parasitology·D E K Tarr, Alan L Scott
Mar 10, 2005·Journal of Molecular Biology·Richard P GrantMurray Stewart
Nov 6, 2007·Current Biology : CB·Maria Ana Gomez-FerreriaDavid J Sharp
Jan 30, 2008·Biophysical Journal·Mark ZajacCharles W Wolgemuth

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