28-Day hindlimb unweighting reduces expression of Rho kinase and inhibits its effects in femoral artery of rat

Journal of Physiology and Biochemistry
Zhong-Chao WangJin Ma

Abstract

Previous studies have demonstrated inconsistent roles of Rho kinase (ROCK) in the decreased vasoconstriction of rat hindquarter vessels induced by hindlimb unweighting (HU). The present study was designed to determine the unclear role of ROCK in the mediation of HU-induced decreased femoral arterial vasoconstriction. 28-day HU rat was adopted as the animal model. With or without Y-27632, a ROCK inhibitor, isometric force of femoral artery was measured. The expression of ROCK and its effects on downstream targets were also examined. Results showed that (1) HU caused a significant decrease of the phenylephrine (PE)-evoked and potassium chloride (KCl)-evoked femoral arterial vasoconstriction (P < 0.05), confirming the functional findings by previous studies. (2) Inhibition of ROCK with Y-27632 produced an equal reduction of the vasoconstriction in CON and HU. (3) HU significantly decreased ROCK II expression and the effects of ROCK on myosin light-chain phosphatase (MLCP) and MLC (P < 0.05), but increased p65 nuclear translocation (P < 0.05) and inducible nitric oxide synthase (iNOS) expression (P < 0.05). (4) HU significantly (P < 0.05) increased NO production in femoral arteries, with Y-27632 significantly (P < 0.01) amplifying ...Continue Reading

References

Jun 23, 1997·FEBS Letters·S NarumiyaN Watanabe
Oct 7, 1998·Journal of Applied Physiology·R E PurdyD Sara
Jun 16, 2001·Journal of Applied Physiology·G Pfitzer
Sep 1, 1981·Acta Astronautica·O G GazenkoA D Egorov
Nov 22, 2001·Journal of Applied Physiology·L F Zhang
Mar 16, 2002·Journal of Applied Physiology·Emily R Morey-Holton, Ruth K Globus
May 15, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Zhao-Jun FuJin Ma
Jan 1, 2005·Journal of Applied Physiology·Natalia I GokinaGeorge Osol
Feb 9, 2005·Hypertension·Takeshi KandaTakao Saruta
Oct 18, 2005·Journal of Applied Physiology·Philippe P ArbeilleDania M Mohty
Feb 14, 2006·American Journal of Physiology. Cell Physiology·Kensuke NomaJames K Liao
Feb 18, 2006·Circulation Research·Gervaise LoirandPierre Pacaud
Mar 21, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Patrick N ColleranMichael D Delp
May 7, 2008·Vascular Pharmacology·Scott M SummersRalph E Purdy
Sep 2, 2008·American Journal of Physiology. Heart and Circulatory Physiology·P ArbeilleR Hughson
Sep 17, 2009·Current Pharmaceutical Design·Qian Zhou, James K Liao
Feb 16, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Steven G DennissJames W E Rush
Sep 3, 2010·Nature Reviews. Cardiology·Gervaise Loirand, Pierre Pacaud
Mar 5, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Curtis R TaylorMichael D Delp

❮ Previous
Next ❯

Citations

Jul 19, 2017·Pflügers Archiv : European journal of physiology·Toshio Kitazawa, Kazuyo Kitazawa
Mar 13, 2018·Canadian Journal of Physiology and Pharmacology·Min JiangJin Ma
Sep 19, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yue WuXianyang Zhu
Mar 2, 2019·In Vitro Cellular & Developmental Biology. Animal·Banglian DengJun Chen
Nov 25, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Qiang LyuJin Ma

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.