2D and 3D cell cultures - a comparison of different types of cancer cell cultures

Archives of Medical Science : AMS
Marta KapałczyńskaKatarzyna Lamperska

Abstract

Cell culture is a widely used in vitro tool for improving our understanding of cell biology, tissue morphology, and mechanisms of diseases, drug action, protein production and the development of tissue engineering. Most research regarding cancer biology is based on experiments using two-dimensional (2D) cell cultures in vitro. However, 2D cultures have many limitations, such as the disturbance of interactions between the cellular and extracellular environments, changes in cell morphology, polarity, and method of division. These disadvantages led to the creation of models which are more closely able to mimic conditions in vivo. One such method is three-dimensional culture (3D). Optimisation of the culture conditions may allow for a better understanding of cancer biology and facilitate the study of biomarkers and targeting therapies. In this review, we compare 2D and 3D cultures in vitro as well as different versions of 3D cultures.

Citations

Apr 5, 2019·Journal of Cellular Physiology·Nipha ChaicharoenaudomrungParinya Noisa
Oct 3, 2019·World Journal of Microbiology & Biotechnology·Duygu Alp, Hakan Kuleaşan
Jul 3, 2019·Molecular Neurobiology·Mirza Ali Mofazzal JahromiMichael R Hamblin
Mar 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Camila HernandesSergio Podgaec
Jul 3, 2019·Clinical and Translational Medicine·Tovah WilliamsonHossein Sendi
Sep 11, 2019·Biophysics Reviews·Ece BayirYannis F Missirlis
Sep 17, 2020·Biotechnology Letters·Qiao Chen, Youbin Wang
May 26, 2018·Nanotechnology·Yimin WangShuQi Wang
Jul 16, 2020·Materials·Liubov BakhchovaUlrike Steinmann
Sep 3, 2020·International Journal of Molecular Sciences·Kamila BiałkowskaKatarzyna Miłowska
Mar 27, 2020·Frontiers in Molecular Biosciences·Caleb Jensen, Yong Teng
Aug 6, 2020·International Journal of Molecular Sciences·Hannah L SmithJanos M Kanczler
Apr 27, 2019·Frontiers in Neuroscience·Dmitry KireevAndreas Offenhäusser
Aug 6, 2020·Frontiers in Bioengineering and Biotechnology·Dinesh DhamechaJyothi U Menon
Feb 12, 2020·Journal of Personalized Medicine·Chiara ArgentatiSabata Martino
Aug 17, 2020·International Journal of Molecular Sciences·Marián MerinoAna Virginia Sánchez-Sánchez
Oct 10, 2020·World Journal of Stem Cells·Cornelia Larissa Granz, Ali Gorji
Jun 24, 2020·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Luke Maggs, Soldano Ferrone
Dec 22, 2020·Frontiers in Oncology·Barbora PavlatovskáJarmila Navrátilová
Jan 21, 2021·Cancers·Guillermo Bordanaba-FloritFélix Royo
Dec 19, 2020·Nanomaterials·Gerardo A Lopez-MuñozJavier Ramón-Azcón
Jan 2, 2021·International Journal of Molecular Sciences·Daniel N SilvaAndré F Moreira
Jan 21, 2021·International Journal of Molecular Sciences·Georgia PennarossaTiziana A L Brevini
Oct 23, 2020·Journal of Personalized Medicine·Gizem Damla YalcinAhmet Acar
Nov 5, 2020·International Journal of Molecular Sciences·Masato OokaMenghang Xia
Dec 20, 2020·Biology·Nicolas GermainPhilippe Marchetti
Feb 16, 2021·Frontiers in Bioengineering and Biotechnology·Chrysanthi-Maria MoysidouRóisín Meabh Owens
Jan 1, 2021·BioMed Research International·Heshu Sulaiman RahmanNoorjahan Banu Alitheen
Jan 6, 2021·International Journal of Molecular Sciences·Tanya SinghSanaz Memarzadeh

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Methods Mentioned

BETA
xenograft
transfection
fluorescence microscopy

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