Abstract
Adipose tissue plays a key role in the development of obesity and diabetes. Natural products are one of the main sources for discovering new lead compounds. In the present study, (2S)-7,4'-dihydroxy-8-prenylflavan (DHPF), a natural prenylated flavan isolated from Morus yunnanensis, was found to significantly promote adipogenesis and increase glucose uptake in 3T3-L1 cells. Real-time PCR results showed that DHPF increased the expression of glucose and lipid metabolism-related genes (C/EBPα, PPARγ, aP2, GLUT4 and adiponectin) and decreased the expression of inflammatory cytokine TNF-α. Western blotting further revealed that DHPF activated p38 MAPK at the initial stage of 3T3-L1 preadipocyte differentiation. DHPF-induced activation of p38, adipogenesis and glucose uptake were effectively blocked by SB203580, a specific p38 inhibitor. These results indicate that DHPF could stimulate adipogenesis and increase glucose uptake through the p38 MAPK pathway, and DHPF may be useful for the prevention and treatment of obesity-associated disorders such as type 2 diabetes (T2D).
References
Mar 1, 1991·The Journal of Clinical Investigation·W T GarveyT P Ciaraldi
May 12, 1988·Nature·D E JamesP F Pilch
Nov 21, 1998·The Journal of Biological Chemistry·J A EngelmanP E Scherer
Aug 23, 2000·The Journal of Clinical Investigation·B B Kahn, J S Flier
Oct 30, 2001·The Proceedings of the Nutrition Society·S W Coppack
Sep 25, 2002·The Journal of Biological Chemistry·Deepanwita PrustyStephen R Farmer
Jun 27, 2003·Drugs·Michaela Diamant, Robert J Heine
Aug 23, 2003·Endocrinology·Michael W Rajala, Philipp E Scherer
Dec 10, 2003·Circulation·Richard W NestoUNKNOWN American Diabetes Association
Jun 26, 2004·Journal of Natural Products·Somdej KanokmedhakulPalangpon Kongsaeree
Sep 26, 2006·Nature Reviews. Immunology·Herbert Tilg, Alexander R Moschen
Oct 3, 2006·Cell Metabolism·Stephen R Farmer
Apr 11, 2008·Nature Reviews. Molecular Cell Biology·Adilson GuilhermeMichael P Czech
Nov 11, 2008·Archives of Medical Research·Maria Eugenia Frigolet Vázquez-VelaArmando R Tovar
May 18, 2010·Biochemical and Biophysical Research Communications·Xiaodong ZhangHeyao Wang
Sep 29, 2011·Nature Reviews. Molecular Cell Biology·Ana G Cristancho, Mitchell A Lazar
Dec 15, 2011·Journal of Natural Products·Xiao HuAi-Jun Hou
Mar 7, 2012·Pharmacoepidemiology and Drug Safety·Marloes T BazelierFrank de Vries
Jun 13, 2012·Diabetologia·A T Turer, P E Scherer
Citations
May 21, 2016·Frontiers in Cell and Developmental Biology·Eddie Rodríguez-CarballoFrancesc Ventura
Jun 16, 2018·International Journal of Molecular Sciences·Ayumu TakenoToshitsugu Sugimoto
Mar 3, 2017·PloS One·Qian ZhaoHaiyang Yu
Jul 23, 2016·Molecular and Cellular Biochemistry·Luoyan AiZhuping Fan
Dec 23, 2016·Endocrine·Jing-Jie ZhuHe-Yao Wang
Dec 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Motohiko UkiyaAtsuyoshi Nishina
Jul 25, 2019·Journal of Agricultural and Food Chemistry·Peng SunHe-Yao Wang