DOI: 10.1101/514174Jan 9, 2019Paper

3'-5' crosstalk contributes to transcriptional bursting

BioRxiv : the Preprint Server for Biology
Massimo CavallaroDaniel Hebenstreit


Transcription in mammalian cells is a complex stochastic process involving shuttling of polymerase between genes and phase-separated liquid condensates. It occurs in bursts, which results in vastly different numbers of an mRNA species in isogenic cell populations. Several factors contributing to "transcriptional bursting" have been identified, usually classified as intrinsic, i.e., local to single genes, or extrinsic, relating to the macroscopic state of the cell. However, each factor only accounts partially for the observed phenomenon, and some possible contributors have not been explored yet. We focussed on processes at the 3' and 5' ends of a gene that enable reinitiation of transcription upon termination. Using Bayesian methodology, we measured the transcriptional bursting in inducible transgenes, showing that perturbation of polymerase shuttling typically reduces burst size, increases burst frequency, and thus limits transcriptional noise. Analysis based on paired-end tag sequencing (ChIA-PET) suggests that this effect is genome wide. The observed noise patterns are also reproduced by a generative model that captures major characteristics of the polymerase flux between a gene and a phase-separated compartment.

Related Concepts

RNA, Messenger
Transcription, Genetic
Isogeneic Graft
Intercellular Communication Process
Population Group

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