DOI: 10.1101/514174Jan 9, 2019Paper

3'-5' crosstalk contributes to transcriptional bursting

BioRxiv : the Preprint Server for Biology
Massimo CavallaroDaniel Hebenstreit

Abstract

Transcription in mammalian cells is a complex stochastic process involving shuttling of polymerase between genes and phase-separated liquid condensates. It occurs in bursts, which results in vastly different numbers of an mRNA species in isogenic cell populations. Several factors contributing to "transcriptional bursting" have been identified, usually classified as intrinsic, i.e., local to single genes, or extrinsic, relating to the macroscopic state of the cell. However, each factor only accounts partially for the observed phenomenon, and some possible contributors have not been explored yet. We focussed on processes at the 3' and 5' ends of a gene that enable reinitiation of transcription upon termination. Using Bayesian methodology, we measured the transcriptional bursting in inducible transgenes, showing that perturbation of polymerase shuttling typically reduces burst size, increases burst frequency, and thus limits transcriptional noise. Analysis based on paired-end tag sequencing (ChIA-PET) suggests that this effect is genome wide. The observed noise patterns are also reproduced by a generative model that captures major characteristics of the polymerase flux between a gene and a phase-separated compartment.

Related Concepts

Genes
RNA, Messenger
Transcription, Genetic
Transgenes
Isogeneic Graft
Patterns
Size
Intercellular Communication Process
Population Group
Polymerase

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

© 2021 Meta ULC. All rights reserved