3-D illustration of network orientations of interstitial cells of Cajal subgroups in human colon as revealed by deep-tissue imaging with optical clearing.

American Journal of Physiology. Gastrointestinal and Liver Physiology
Yuan-An LiuShiue-Cheng Tang

Abstract

Morphological changes of interstitial cells of Cajal (ICC) have been proposed to characterize motility disorders. However, a global view of the network orientations of ICC subgroups has not been established to illustrate their three-dimensional (3-D) architectures in the human colon. In this research, we integrate c-kit immunostaining, 3-D microscopy with optical clearing, and image rendering to present the location-dependent network orientations with high definition. Full-depth colonic tissues were obtained from colectomies performed for nonobstructing carcinoma. Specimens of colon wall were prepared away from the tumor site. C-kit and nuclear fluorescent staining were used to identify the ICC processes and cell body. Optical clearing was used to generate transparent colon specimens, which led to panoramic visualization of the fluorescence-labeled ICC networks at the myenteric plexus (ICC-MY), longitudinal (ICC-LM) and circular (ICC-CM) muscles, and submucosal boundary (ICC-SM) up to 300 μm in depth via confocal microscopy with subcellular level resolution. We observed four distinct network patterns: 1) periganglionic ICC-MY that connect with ICC-LM and ICC-CM, 2) plexuses of ICC-LM within the longitudinal muscle and extending...Continue Reading

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Citations

Jun 27, 2013·Diabetologia·Shiue-Cheng TangYa-Yuan Fu
Aug 7, 2012·Cell and Tissue Research·Peter J BlairSean M Ward
Jun 28, 2013·Journal of the Royal Society, Interface·Jerry GaoLeo K Cheng
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Mar 19, 2013·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Y A LiuS C Tang
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Apr 27, 2021·American Journal of Physiology. Endocrinology and Metabolism·Chien-Chia ChenShiue-Cheng Tang

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