3-D imaging and illustration of mouse intestinal neurovascular complex

American Journal of Physiology. Gastrointestinal and Liver Physiology
Ya-Yuan FuShiue-Cheng Tang

Abstract

Because of the dispersed nature of nerves and blood vessels, standard histology cannot provide a global and associated observation of the enteric nervous system (ENS) and vascular network. We prepared transparent mouse intestine and combined vessel painting and three-dimensional (3-D) neurohistology for joint visualization of the ENS and vasculature. Cardiac perfusion of the fluorescent wheat germ agglutinin (vessel painting) was used to label the ileal blood vessels. The pan-neuronal marker PGP9.5, sympathetic neuronal marker tyrosine hydroxylase (TH), serotonin, and glial markers S100B and GFAP were used as the immunostaining targets of neural tissues. The fluorescently labeled specimens were immersed in the optical clearing solution to improve photon penetration for 3-D confocal microscopy. Notably, we simultaneously revealed the ileal microstructure, vasculature, and innervation with micrometer-level resolution. Four examples are given: 1) the morphology of the TH-labeled sympathetic nerves: sparse in epithelium, perivascular at the submucosa, and intraganglionic at myenteric plexus; 2) distinct patterns of the extrinsic perivascular and intrinsic pericryptic innervation at the submucosal-mucosal interface; 3) different ass...Continue Reading

References

Jun 1, 1994·Journal of the Autonomic Nervous System·J D Wood
Aug 1, 1996·The American Journal of Physiology·S Vanner, A Surprenant
Nov 5, 1997·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·H J CookeY Z Wang
Aug 29, 1998·Journal of the Autonomic Nervous System·M HananiJ H Szurszewski
Jan 28, 2003·The American Journal of Surgical Pathology·Benjamin C CalhounDhanpat Jain
Mar 21, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·David R LindenGary M Mawe
Jul 1, 1955·Physiological Reviews·B FOLKOW
Aug 13, 2005·Microvascular Research·Dino J RavnicMoritz A Konerding
Apr 25, 2006·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·K KorenagaP J Pasricha
Feb 27, 2008·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·D BirchG Burnstock
May 10, 2008·Journal of Biomedical Optics·Valery V TuchinAlvin T Yeh
Aug 22, 2009·European Journal of Gastroenterology & Hepatology·Bernard DenisJean François Fléjou
Feb 13, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·C A CobineK D Keef
Aug 24, 2010·Gastroenterology·Ya-Yuan Fu, Shiue-Cheng Tang
Jun 28, 2011·Gastroenterology·Gerard Karsenty, Michael D Gershon
Sep 8, 2011·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Y-A LiuS-C Tang
Oct 6, 2011·Nature Reviews. Gastroenterology & Hepatology·Katherine Smith
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yasmine Chabbi-AchengliMarie-Christine de Vernejoul

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Citations

May 23, 2014·Cellular and Molecular Life Sciences : CMLS·Sergio Britto GarciaVinicius Kannen
Jun 27, 2013·Diabetologia·Shiue-Cheng TangYa-Yuan Fu
Feb 18, 2016·Histochemistry and Cell Biology·Douglas J Taatjes, Jürgen Roth
Sep 10, 2014·Diabetes, Obesity & Metabolism·S-C TangH-J Chien
Oct 27, 2015·International Journal of Obesity : Journal of the International Association for the Study of Obesity·H-J ChienS-C Tang
Mar 19, 2013·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Y A LiuS C Tang
Jul 2, 2015·Journal of Neurogastroenterology and Motility·Werend BoesmansPieter Vanden Berghe
Jul 23, 2013·Organogenesis·Léa M M CostesCathy Cailotto
Nov 23, 2013·Developmental Biology·Jean-Marie DelalandeAlan J Burns
Nov 6, 2014·Histochemistry and Cell Biology·Sandra SchrenkKarl-Herbert Schäfer
Feb 11, 2015·The Journal of Clinical Investigation·Marina AvetisyanRobert O Heuckeroth
Apr 20, 2017·Proceedings of the National Academy of Sciences of the United States of America·Subhash KulkarniPankaj J Pasricha
Jun 29, 2017·Nature Reviews. Gastroenterology & Hepatology·Jeremiah Bernier-Latmani, Tatiana V Petrova
Mar 9, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Camille PochardMalvyne Rolli-Derkinderen
Aug 16, 2018·Physiological Reviews·Colin ReardonAlan E Lomax
Sep 3, 2017·Diabetologia·Shiue-Cheng TangPankaj J Pasricha
Dec 29, 2015·Diabetes & Metabolism Journal·Heung Yong JinTae Sun Park
Feb 15, 2019·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Gleison D P BossolaniJean-Pierre Timmermans
Aug 26, 2016·Nature Protocols·Jeremiah Bernier-Latmani, Tatiana V Petrova
Jan 16, 2014·American Journal of Physiology. Endocrinology and Metabolism·Jyuhn-Huarng JuangShiue-Cheng Tang
Aug 12, 2014·Journal of Applied Physiology·Mi-Kyung ShinVsevolod Y Polotsky
May 19, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Cambrian Y Liu, D Brent Polk
Nov 9, 2018·BioMed Research International·Pollyana Ribeiro CastroLuciola Silva Barcelos
Sep 12, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hung-Jen ChienShiue-Cheng Tang
Apr 7, 2020·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Retsu Mitsui, Hikaru Hashitani
Dec 10, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Anne S Ten HoveWouter J de Jonge
Dec 17, 2020·ACS Chemical Neuroscience·Jiajia ZhaoHaitao Sun
Sep 13, 2021·Cellular and Molecular Gastroenterology and Hepatology·Andrea Nestor-KalinoskiMarthe J Howard
Dec 6, 2021·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Wael N El-NachefMarianne E Bronner

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