3-D imaging of islets in obesity: formation of the islet-duct complex and neurovascular remodeling in young hyperphagic mice

International Journal of Obesity : Journal of the International Association for the Study of Obesity
H-J ChienS-C Tang

Abstract

Obesity and insulin resistance lead to islet hyperplasia. However, how the islet remodeling influences the pancreatic environment and the associated neurovascular networks is largely unknown. The lack of information is primarily due to the difficulty of global visualization of the hyperplasic islet (>200 μm) and the neurovascular environment with high definition. We modulated the pancreatic optical property to achieve 3-dimensional (3-D) whole-islet histology and to integrate transmitted light microscopy (which provides the ground-truth tissue information) with confocal fluorescence imaging. The new optical and imaging conditions were used to globally examine the hyperplastic islets of the young (2 months) obese db/db and ob/ob mice, which otherwise cannot be easily portrayed by the standard microtome-based histology. The voxel-based islet micrographs were digitally processed for stereo projection and qualitative and quantitative analyses of the islet tissue networks. Paired staining and imaging of the pancreatic islets, ducts and neurovascular networks reveal the unexpected formation of the 'neuro-insular-ductal complex' in the young obese mice. The complex consists of the peri- and/or intra-islet ducts and prominent peri-duct...Continue Reading

References

Jul 1, 1974·Physiological Reviews·S C Woods, D Porte
Mar 1, 1996·Diabetes Care·D GiuglianoG Paolisso
May 2, 2001·The Journal of Cell Biology·M HellströmC Betsholtz
Oct 9, 2002·Circulation·John P Cooke, Roberta K Oka
May 9, 2003·American Journal of Physiology. Renal Physiology·Kumar SharmaStephen R Dunn
Aug 6, 2004·Endocrine Reviews·Andrea M VincentEva L Feldman
Mar 29, 2005·Diabetes Care·Andrew J M BoultonUNKNOWN American Diabetes Association
Oct 11, 2005·Neuro-oncology·Gabriele Bergers, Steven Song
Feb 18, 2006·The Journal of Clinical Investigation·Haruka OkamotoDomenico Accili
Sep 7, 2007·Neurobiology of Disease·Kelli A SullivanEva L Feldman
Aug 19, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Wen SuMing C Gong
Oct 7, 2009·International Journal of Obesity : Journal of the International Association for the Study of Obesity·G SikkaL A Barouch
Oct 13, 2009·Diabetes, Obesity & Metabolism·C TalchaiD Accili
Feb 4, 2010·European Child & Adolescent Psychiatry·Anke HinneyJohannes Hebebrand
Nov 6, 2010·Expert Review of Medical Devices·Elina A GeninaValery V Tuchin
Jan 29, 2011·Journal of Biomedicine & Biotechnology·Keizo Kanasaki, Daisuke Koya
May 6, 2011·Diabetes Care·Ebe D'Adamo, Sonia Caprio
Nov 9, 2011·Nature Reviews. Endocrinology·Lei ChenPaul Z Zimmet
Dec 14, 2011·International Journal of Obesity : Journal of the International Association for the Study of Obesity·J R FernandezK Casazza
Mar 17, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yuan-An LiuShiue-Cheng Tang
Oct 23, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ya-Yuan FuShiue-Cheng Tang
Apr 12, 2013·Nature·Kwanghun ChungKarl Deisseroth
Jun 27, 2013·Diabetologia·Shiue-Cheng TangYa-Yuan Fu
Oct 25, 2013·Frontiers in Neuroanatomy·Tzu-En HuaShiue-Cheng Tang
Jan 16, 2014·American Journal of Physiology. Endocrinology and Metabolism·Jyuhn-Huarng JuangShiue-Cheng Tang
Feb 19, 2014·JAMA Pediatrics·Clare H LlewellynJane Wardle
Feb 19, 2014·Diabetologia·Xiangwei XiaoGeorge K Gittes
Apr 18, 2014·Nature·Cassandra Willyard
May 16, 2014·Advances in Nutrition·Amelia K LinnemannDawn Belt Davis

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Citations

Feb 8, 2018·Frontiers in Neuroanatomy·Xuefeng YuanLaurent Gautron
Jun 25, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Pei-Yu LinShiue-Cheng Tang
Sep 3, 2017·Diabetologia·Shiue-Cheng TangPankaj J Pasricha
Sep 27, 2018·Advances in Anatomic Pathology·Seung-Mo HongRalph H Hruban
Apr 3, 2020·Journal of Molecular Cell Biology·Jinglong Guo, Wenxian Fu
Sep 12, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hung-Jen ChienShiue-Cheng Tang
Aug 31, 2017·Diabetologia·Shiue-Cheng TangMichael S German
Oct 11, 2020·Science Advances·Alexandra AlvarssonSarah A Stanley
Apr 27, 2021·American Journal of Physiology. Endocrinology and Metabolism·Chien-Chia ChenShiue-Cheng Tang
May 14, 2021·Frontiers in Endocrinology·Martha Campbell-Thompson, Shiue-Cheng Tang

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