3-D imaging of islets in obesity: formation of the islet-duct complex and neurovascular remodeling in young hyperphagic mice

International Journal of Obesity : Journal of the International Association for the Study of Obesity
H-J ChienS-C Tang


Obesity and insulin resistance lead to islet hyperplasia. However, how the islet remodeling influences the pancreatic environment and the associated neurovascular networks is largely unknown. The lack of information is primarily due to the difficulty of global visualization of the hyperplasic islet (>200 μm) and the neurovascular environment with high definition. We modulated the pancreatic optical property to achieve 3-dimensional (3-D) whole-islet histology and to integrate transmitted light microscopy (which provides the ground-truth tissue information) with confocal fluorescence imaging. The new optical and imaging conditions were used to globally examine the hyperplastic islets of the young (2 months) obese db/db and ob/ob mice, which otherwise cannot be easily portrayed by the standard microtome-based histology. The voxel-based islet micrographs were digitally processed for stereo projection and qualitative and quantitative analyses of the islet tissue networks. Paired staining and imaging of the pancreatic islets, ducts and neurovascular networks reveal the unexpected formation of the 'neuro-insular-ductal complex' in the young obese mice. The complex consists of the peri- and/or intra-islet ducts and prominent peri-duct...Continue Reading


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Feb 8, 2018·Frontiers in Neuroanatomy·Xuefeng YuanLaurent Gautron
Jun 25, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Pei-Yu LinShiue-Cheng Tang
Sep 3, 2017·Diabetologia·Shiue-Cheng TangPankaj J Pasricha
Sep 27, 2018·Advances in Anatomic Pathology·Seung-Mo HongRalph H Hruban
Apr 3, 2020·Journal of Molecular Cell Biology·Jinglong Guo, Wenxian Fu
Sep 12, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hung-Jen ChienShiue-Cheng Tang
Aug 31, 2017·Diabetologia·Shiue-Cheng TangMichael S German
Oct 11, 2020·Science Advances·Alexandra AlvarssonSarah A Stanley
Apr 27, 2021·American Journal of Physiology. Endocrinology and Metabolism·Chien-Chia ChenShiue-Cheng Tang
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