3-D modelling of chloroplast structure under (Mg2+) magnesium ion treatment. Relationship between thylakoid membrane arrangement and stacking

Biochimica Et Biophysica Acta
Izabela RumakMaciej Garstka

Abstract

We performed for the first time three-dimensional (3D) modelling of the entire chloroplast structure. Stacks of optical slices obtained by confocal laser scanning microscope (CLSM) provided a basis for construction of 3D images of individual chloroplasts. We selected pea (Pisum sativum) and bean (Phaseolus vulgaris) chloroplasts since we found that they differ in thylakoid organization. Pea chloroplasts contain large distinctly separated appressed domains while less distinguished appressed regions are present in bean chloroplasts. Different magnesium ion treatments were used to study thylakoid membrane stacking and arrangement. In pea chloroplasts, as demonstrated by 3D modelling, the increase of magnesium ion concentration changed the degree of membrane appression from wrinkled continuous surface to many distinguished stacked areas and significant increase of the inter-grana area. On the other hand 3D models of bean chloroplasts exhibited similar but less pronounced tendencies towards formation of appressed regions. Additionally, we studied arrangements of thylakoid membranes and chlorophyll-protein complexes by various spectroscopic methods, Fourier-transform infrared spectroscopy (FTIR) among others. Based on microscopic and...Continue Reading

References

Jun 23, 1998·Quarterly Reviews of Biophysics·L K Tamm, S A Tatulian
Jul 7, 1999·Biochimica Et Biophysica Acta·E GoormaghtighJ M Ruysschaert
Nov 14, 2001·Journal of Photochemistry and Photobiology. B, Biology·M Garstka, A Jagielski
Mar 29, 2003·Trends in Plant Science·László Mustárdy, Gyozo Garab
Sep 26, 2003·Biochimica Et Biophysica Acta·Andrei BorodichGunnar Oquist
Nov 10, 2004·Biochemistry·Helmut KirchhoffClaudia Büchel
Dec 29, 2004·Biochimica Et Biophysica Acta·Jan P Dekker, Egbert J Boekema
Jun 15, 2005·Biochimica Et Biophysica Acta·Eun-Ha KimJan M Anderson
Jan 13, 2006·Journal of Photochemistry and Photobiology. B, Biology·Katerina StoitchkovaAtanaska Andreeva
May 30, 2006·Biochimica Et Biophysica Acta·Jon Nield, James Barber
Sep 19, 2006·Biochimica Et Biophysica Acta·Wieslaw I GruszeckiWaldemar Maksymiec
Jul 10, 2007·Biochimica Et Biophysica Acta·Helmut KirchhoffMatthias Rögner
Feb 22, 2008·Plant Physiology·Helmut KirchhoffConrad W Mullineaux
Feb 29, 2008·Biochemistry·Fikret MamedovStenbjörn Styring
Apr 10, 2008·The Plant Cell·Silvia G ChuartzmanZiv Reich
Apr 15, 2008·Trends in Plant Science·Helmut Kirchhoff
Oct 28, 2008·The Plant Cell·Vlad BrumfeldZiv Reich

❮ Previous
Next ❯

Citations

Jun 17, 2011·Photosynthesis Research·Dalton CarmelEva-Mari Aro
Jun 25, 2013·Journal of Photochemistry and Photobiology. B, Biology·Ewa JanikWaldemar Maksymiec
Apr 3, 2012·Biochimica Et Biophysica Acta·Lucja RudowskaAgnieszka Mostowska
May 25, 2011·Biochimica Et Biophysica Acta·Mikko Tikkanen, Eva-Mari Aro
Jan 1, 2015·Nature Plants·Alexander V Ruban, Matthew P Johnson
Jan 5, 2019·Journal of Bioenergetics and Biomembranes·Nia PetrovaSashka Krumova
Dec 27, 2016·Frontiers in Plant Science·Radek Kaňa, Govindjee
Sep 1, 2017·Scientific Reports·Pascal AlbaneseCristina Pagliano
May 1, 2018·Functional Plant Biology : FPB·Izabela A SamborskaAnjana Jajoo
Mar 10, 2021·Nature Plants·Derren J HeyesNigel S Scrutton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.