3-D physiomimetic extracellular matrix hydrogels provide a supportive microenvironment for rodent and human islet culture

Biomaterials
K JiangC L Stabler

Abstract

Organ-on-a-chip platforms serve as cost-efficient testbeds for screening pharmaceutical agents, mimicking natural physiology, and studying disease. In the field of diabetes, the development of an islet-on-a-chip platform would have broad implications in understanding disease pathology and discovering potential therapies. Islet microphysiological systems are limited, however, by their poor cell survival and function in culture. A key factor that has been implicated in this decline is the disruption of islet-matrix interactions following isolation. Herein, we sought to recapitulate the in vivo peri-islet niche using decellularized extracellular matrix (ECM) hydrogels. Sourcing from porcine bladder, lung, and pancreas tissues, 3-D ECM hydrogels were generated, characterized, and validated using both rodent and human pancreatic islets. Optimized decellularization protocols resulted in hydrogels with distinctive viscoelastic properties that correlated to their matrix composition. The in situ 3-D encapsulation of human or rat islets within ECM hydrogels resulted in improved functional stability over standard culture conditions. Islet composition and morphology were also altered, with enhanced retention of islet-resident endothelial c...Continue Reading

Citations

Nov 7, 2018·Tissue Engineering. Part C, Methods·Roberto GaetaniKaren L Christman
Dec 12, 2019·Journal of Tissue Engineering and Regenerative Medicine·Claudia BilodeauMartin Post
Apr 24, 2020·Science Translational Medicine·Kristina G MaxwellJeffrey R Millman
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Aug 15, 2020·Frontiers in Bioengineering and Biotechnology·Chizhu DingXianghua Yan
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Feb 4, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mijung JungChang Seok Ki
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Apr 26, 2021·Biomaterials·Jorge Santini-GonzálezEdward A Phelps

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