3-D visualization and quantitation of microvessels in transparent human colorectal carcinoma [corrected

PloS One
Yuan-An LiuYuan-Chiang Chung

Abstract

Microscopic analysis of tumor vasculature plays an important role in understanding the progression and malignancy of colorectal carcinoma. However, due to the geometry of blood vessels and their connections, standard microtome-based histology is limited in providing the spatial information of the vascular network with a 3-dimensional (3-D) continuum. To facilitate 3-D tissue analysis, we prepared transparent human colorectal biopsies by optical clearing for in-depth confocal microscopy with CD34 immunohistochemistry. Full-depth colons were obtained from colectomies performed for colorectal carcinoma. Specimens were prepared away from (control) and at the tumor site. Taking advantage of the transparent specimens, we acquired anatomic information up to 200 μm in depth for qualitative and quantitative analyses of the vasculature. Examples are given to illustrate: (1) the association between the tumor microstructure and vasculature in space, including the perivascular cuffs of tumor outgrowth, and (2) the difference between the 2-D and 3-D quantitation of microvessels. We also demonstrate that the optically cleared mucosa can be retrieved after 3-D microscopy to perform the standard microtome-based histology (H&E staining and immun...Continue Reading

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Citations

Dec 4, 2014·Cellular and Molecular Gastroenterology and Hepatology·Yuan-An LiuShiue-Cheng Tang
Feb 28, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Cambrian Y LiuD Brent Polk
Sep 10, 2014·Diabetes, Obesity & Metabolism·S-C TangH-J Chien
Oct 21, 2020·Scientific Reports·Inna Sabdyusheva LitschauerHans-Ulrich Dodt
Aug 1, 2021·Nature Reviews. Cancer·Jorge AlmagroAxel Behrens

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Methods Mentioned

BETA
biopsy
light scattering
light microscopy
biopsies
confocal microscopy

Software Mentioned

Zeiss Zen
LSM 510
Avizo
Amira
Linux
Zen

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